Increased cellulose production from sucrose with reduced levan accumulation by an Acetobacter strain harboring a recombinant plasmid

Bioscience, Biotechnology, and Biochemistry
N TonouchiS Horinouchi

Abstract

Cellulose production from sucrose by Acetobacter strains is accompanied by the accumulation of a water-soluble polysaccharide, called levan. To improve cellulose productivity, a levansucrase-deficient mutant, LD-2, was derived from Acetobacter strain 757 and used as a host for the construction of recombinant strains. An LD-2 mutant harboring a plasmid containing the sucrase gene, sucZE3, from Zymomonas mobilis together with zliS, a gene that encodes a secretion-activating factor under the control of the Escherichia coli lac promoter, had sucrase activity and produced much cellulose and little levan in a medium containing sucrose. In addition, a mutant levansucrase gene, mutant sacB, from Bacillus subtilis, which encodes a protein with little levan-forming activity, was generated by site-directed mutagenesis and introduced into the LD-2 mutant. This introduction also resulted in the higher cellulose productivity and little levan.

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H C WongA W Emerick

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Citations

Sep 13, 2007·Critical Reviews in Biotechnology·Cassandra De MuynckErick J Vandamme
Jan 1, 1998·Bioscience, Biotechnology, and Biochemistry·N TonouchiF Yoshinaga
Sep 13, 2002·Bioscience, Biotechnology, and Biochemistry·Toshio ShidaJunichi Sekiguchi
Mar 7, 2003·Bioscience, Biotechnology, and Biochemistry·Naoto TonouchiKenzo Yokozeki
Apr 16, 2021·Applied Microbiology and Biotechnology·Philipp Moritz FrickeTino Polen

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