Increased circulating microRNA-122 is a biomarker for discrimination and risk stratification in patients defined by sepsis-3 criteria

PloS One
Tim RahmelJürgen Peters

Abstract

Sepsis is now operationally defined as life-threatening organ dysfunction caused by an infection, identified by an acute change in SOFA-Score of at least two points, including clinical chemistry such as creatinine or bilirubin concentrations. However, little knowledge exists about organ-specific microRNAs as potentially new biomarkers. Accordingly, we tested the hypotheses that micro-RNA-122, the foremost liver-related micro-RNA (miR), 1) discriminates between sepsis and infection, 2) is an early predictor for mortality, and 3) improves the prognostic value of the SOFA-score. We analyzed 108 patients with sepsis (infection + increase SOFA-Score ≥2) within the first 24h of ICU admission and as controls 20 patients with infections without sepsis (infection + SOFA-Score ≤1). Total circulating miR was isolated from serum and relative miR-122 expression was measured (using spiked-in cel-miR-54) and associated with 30-day survival. 30-day survival of the sepsis patients was 63%. miR-122 expression was 40-fold higher in non-survivors (p = 0.001) and increased almost 6-fold in survivors (p = 0.013) compared to controls. miR-122 serum-expression discriminated both between sepsis vs. infection (AUC 0.760, sensitivity 58.3%, specificity 9...Continue Reading

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Citations

Aug 10, 2019·Anesthesia and Analgesia·Ettore CrimiClaudio Napoli
Jul 18, 2020·Intensive Care Medicine Experimental·Clifford S DeutschmanUNKNOWN Research Committee of the Surviving Sepsis Campaign
Jul 23, 2020·Critical Care Medicine·Clifford S DeutschmanUNKNOWN Research Committee of the Surviving Sepsis Campaign
Jun 6, 2020·Frontiers in Pharmacology·Cheng-Kai HuangThomas Thum
Dec 10, 2020·Journal of Intensive Care·Xiaomin ShenYunqing Qiu
May 27, 2021·Critical Reviews in Toxicology·Brian N ChorleyAlison H Harrill
Sep 11, 2021·Journal of Translational Medicine·Jianyuan PanYongqin Tang

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS
R Foundation for
R
GraphPad Prism

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