Increased colon tumor susceptibility in azoxymethane treated CEABAC transgenic mice

Carcinogenesis
Carlos H F ChanC P Stanners

Abstract

Human carcinoembryonic antigen (CEA), a widely used clinical tumor marker, and its close relative, CEACAM6, are often overexpressed in many cancers. This correlation suggests a possible instrumental role in tumorigenesis, which is supported by extensive results obtained with several in vitro systems. The implication that these results could also apply in vivo warrants investigation. Since mice do not possess homologs of the glycophosphatidyl inositol (GPI)-anchored CEACAM family genes CEA, CEACAM6 and CEACAM7, we have constructed transgenic mice harboring a 187 kb portion of the human CEACAM family gene locus contained in a bacterial artificial chromosome (CEABAC) that includes genes coding for CEA, CEACAM6 and CEACAM7. In this study, we treated the CEABAC mice and their wild-type littermates with azoxymethane (AOM) in order to induce colon tumor formation. At 20 weeks post-treatment, the CEABAC transgenics showed more than a 2-fold increase in mean tumor load relative to their wild-type littermates. Cell surface expression of CEA and CEACAM6 increased by 2- and 20-fold, respectively, in colonocytes from the tumors relative to colonocytes from non-AOM treated transgenics and a de-regulated spatial pattern of CEA/CEACAM6 express...Continue Reading

Citations

Aug 2, 2013·Cancer Metastasis Reviews·Nicole Beauchemin, Azadeh Arabzadeh
Apr 13, 2011·Journal of Carcinogenesis·Mariangela De RobertisVito Michele Fazio
Sep 21, 2010·Journal of Physiology and Biochemistry·Lamia LahouarLotfi Achour
Oct 19, 2011·Gastroenterology Research and Practice·Asa HåkanssonSiv Ahrné
Oct 29, 2011·Ultrasound in Medicine & Biology·Kelly Z AlvesJoão C Machado
May 1, 2007·Biomolecular Engineering·David Filpula
Feb 27, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J BoatengC B Chawan
Aug 22, 2006·Current Opinion in Cell Biology·Katharina KuespertChristof R Hauck
Dec 19, 2013·BioMed Research International·Peppino Mirabelli, Mariarosaria Incoronato
Mar 10, 2015·PloS One·Julie M CramerEric Lagasse
Sep 17, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jinfeng ZhouDaiming Fan
Oct 1, 2020·The Journal of International Medical Research·Xinwen ZhangHongbang Xu
Aug 10, 2018·Intestinal research·Linda Chia-Hui YuYen-Hsuan Ni

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