Increased concentrations of apo A-I and apo A-II fragments in the serum of patients with hepatocellular carcinoma by magnetic beads-assisted MALDI-TOF mass spectrometry

American Journal of Clinical Pathology
Yang LiuFumio Nomura

Abstract

Recent advances in sophisticated technologies in proteomics should provide promising ways to discover novel markers for hepatocellular carcinoma (HCC) in the early diagnosis. Serum peptide and protein profiling was conducted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Profiling was carried out in a training set of 16 patients with HCC and a testing set of 15 patients with cirrhosis without HCC. All the patients were hepatitis C virus positive. Candidate peaks were processed to partial purification, followed by protein identification by amino acid sequence analysis. Immunoprecipitation was conducted to confirm the protein identity. Partial purification and protein identification revealed that one peak that was up-regulated in HCC sera both in the training and the testing sets was a fragment of apolipoprotein A-I (apo A-I). Immunoprecipitation confirmed this result. MALDI-TOF MS analysis revealed that apo A-I is a potential novel serum marker of HCC. Combination of these pretreatments and the current magnet bead-assisted MALDI-TOF MS will further enhance the efficiency of biomarker discovery for HCC.

References

Mar 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S L TsaiD S Chen
Aug 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M ShermanC Lee
Jul 4, 2002·Molecular & Cellular Proteomics : MCP·Guoan ChenDavid G Beer
Jan 24, 2003·AJR. American Journal of Roentgenology·Janio SzklarukChusilp Charnsangavej
Feb 4, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Valérie ParadisPierre Bedossa
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Norimasa MiuraGoshi Shiota
May 27, 2005·Seminars in Liver Disease·Morris Sherman
Jun 6, 2006·Cancer·Laura SmithUNKNOWN Cancer Biology Proteomics Group
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Deborah L DiamondMichael G Katze
Mar 3, 2007·Alcoholism, Clinical and Experimental Research·Kazuyuki SogawaFumio Nomura
Mar 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shuji KanmuraHirohito Tsubouchi
Jun 15, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fumio NomuraTatsuya Ohashi
Aug 9, 2007·Advances in Clinical Chemistry·M A Reymond, W Schlegel
Feb 27, 2008·World Journal of Gastroenterology : WJG·Jie-Feng CuiToshimasa Uemura
Mar 7, 2008·World Journal of Gastroenterology : WJG·Kenichi HakamadaMutsuo Sasaki
Jun 24, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Masanori SeimiyaFumio Nomura
Jul 31, 2008·World Journal of Gastroenterology : WJG·Asmaa-Ibrahim GomaaSimon-D Taylor-Robinson
Aug 17, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hiroshi UmemuraFumio Nomura
Jul 1, 2009·Proteomics. Clinical Applications·Kazuyuki SogawaFumio Nomura

❮ Previous
Next ❯

Citations

Mar 8, 2016·Journal of Cancer Research and Clinical Oncology·Hongqing LiWeiping Jiang
Oct 2, 2019·Cancer Medicine·Liwen RenGuanhua Du
Aug 30, 2019·Biomedical Chromatography : BMC·Rabia JaveedMuhammad Najam-Ul-Haq
May 25, 2021·Cancer Biomarkers : Section a of Disease Markers·Varshasnata MohantyAditi Chatterjee
Sep 26, 2014·Journal of Proteome Research·Ilaria InserraClaudia Desiderio

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Related Papers

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Zhi-Hua JiangFei Liu
Rinsho byori. The Japanese journal of clinical pathology
A Noma, M Kita
Rinsho byori. The Japanese journal of clinical pathology
I Sakurabayashi, Y Goto
Nihon rinsho. Japanese journal of clinical medicine
Tsutomu Kazumi, Gen Yoshino
© 2021 Meta ULC. All rights reserved