Abstract
We have studied the cytotoxicity of ricin in a monensin-resistant mutant (MonR-31) of Chinese hamster ovary (CHO) cell line which is presumably altered in Golgi functions/structures. The cytotoxicity of ricin was increased in MonR-31 mutant cells compared with that in its parental CHO cells. In wild-type CHO cells, the cytotoxicity of ricin was enhanced by HN4Cl, bafilomycin A1, or nigericin. The enhancement of ricin cytotoxicity by these compounds was greatly reduced in MonR-31 mutant cells. Brefeldin A (BFA), which disrupts the structure of the Golgi apparatus, inhibits the cytotoxicity of ricin in both CHO and MonR-31 cells. We have also examined the effects of glycosylation inhibitors and the removal of high mannose oligosaccharide chains in ricin on the ricin hypersensitivity in MonR-31 cells. The hypersensitivity of MonR-31 cells to ricin is apparently not due to any difference in glycosylation between CHO and MonR-31 cells or in the processing of oligosaccharides on ricin by the target cells. Nigericin at low concentration (10 nM), which has no effect on the cytotoxicity of diphtheria toxin, enhances the ricin cytotoxicity, but inhibits the modeccin cytotoxicity. Our results suggest that important step(s) in the intoxica...Continue Reading
References
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Jan 1, 1976·Annual Review of Biochemistry·B C Pressman
Mar 10, 1989·Cell·J Lippincott-SchwartzR D Klausner
Jul 1, 1989·The Journal of Cell Biology·R W DomsJ W Yewdell
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E J BowmanK Altendorf
Aug 1, 1985·The Journal of Cell Biology·P C GhoshH C Wu
Jan 1, 1986·The Journal of Cell Biology·B van DeursS Olsnes
Feb 1, 1986·The Biochemical Journal·L FoddyR C Hughes
Feb 1, 1988·Experimental Cell Research·P C Ghosh, H C Wu
Mar 1, 1985·Cell·R G Anderson, R K Pathak
Sep 1, 1981·The Journal of Cell Biology·B Poole, S Ohkuma
Mar 1, 1982·Cell·B Tycko, F R Maxfield
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J GallowayI Mellman
May 1, 1983·Infection and Immunity·A D O'Brien, G D LaVeck
Sep 1, 1983·Infection and Immunity·J M Moehring, T J Moehring
Oct 1, 1984·The Journal of Cell Biology·A R RobbinsI Mellman
Dec 1, 1984·The Journal of Cell Biology·M H MarnellR K Draper
Mar 1, 1984·The Journal of Cell Biology·K SandvigS Olsnes
May 1, 1984·Journal of Cellular Physiology·M OnoM Kuwano
Apr 1, 1982·The Journal of Cell Biology·M H MarnellR K Draper
Dec 1, 1980·The Journal of Cell Biology·R K Draper, M I Simon
Citations
Jul 1, 1996·Journal of Cellular Biochemistry·D D Pless, R B Wellner
Jun 9, 1998·Journal of Cellular Physiology·M P NambiarH C Wu
Jan 1, 1992·Journal of Biochemical Toxicology·S M NaseemJ G Pace
Sep 1, 1993·Journal of Biochemical Toxicology·S M Naseem, J G Pace
Nov 1, 1991·Journal of Cellular Biochemistry·E L MelbyK Sandvig
Feb 1, 1993·Journal of Cellular Physiology·M P NambiarH C Wu
Nov 1, 1993·In Vitro Cellular & Developmental Biology. Animal·S OhkumaH Tamura
Mar 30, 2001·European Journal of Biochemistry·N OlmoM A Lizarbe
Nov 1, 1991·The Journal of Cell Biology·K SandvigB van Deurs
Dec 1, 1993·Molecular Biology of the Cell·L S JohnsonT E McGraw
Apr 1, 1996·Molecular Microbiology·T L Cover
Feb 8, 2002·Cell Structure and Function·I KusanoT Muramatsu
Apr 9, 1998·Bioscience, Biotechnology, and Biochemistry·T OdaT Muramatsu
Sep 3, 2011·PloS One·Supriyo RayKen Teter
Dec 3, 2011·Traffic·Anne Berit Dyve LingelemKirsten Sandvig
Feb 24, 1995·The Journal of Biological Chemistry·T OdaH C Wu
Apr 1, 1993·Infection and Immunity·T L CoverM J Blaser
Dec 1, 1992·Experimental Cell Research·C H ChenH C Wu