Increased drinking in mutant mice with truncated M5 muscarinic receptor genes

Pharmacology, Biochemistry, and Behavior
Junichi TakeuchiJohn Yeomans

Abstract

The rarest and least understood of the muscarinic receptors is the M5 subtype. Recombinant methods were used to create mutant mice with a deletion in the third intracellular loop of the M5 receptor gene. Salivation induced by the nonselective muscarinic agonist pilocarpine (1 mg/kg s.c.) was reduced in homozygous mutants from 15 to 60 min after injection as compared with wild-type mice. After 18-h food and water deprivation, drinking was increased in homozygous mutants, but feeding was not increased. The mutant and wild-type mice had similar responses in tests of open-field exploration, seizures induced by pilocarpine (300 mg/kg) or hypothermia induced by pilocarpine (1-3 mg/kg). These results indicate that M5 muscarinic receptors are important for fluid intake and suggest that M5 receptors are involved in slow secretory processes.

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Citations

Sep 19, 2003·Pharmacology, Biochemistry, and Behavior·Araba ChintohJohn S Yeomans
Jan 8, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·François LaplanteRémi Quirion
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Jürgen Wess
May 18, 2004·The Journal of Physiology·Takeshi NakamuraKatsuhiko Mikoshiba
Feb 24, 2010·Journal of Clinical Pharmacy and Therapeutics·R B Raffa
Oct 12, 2004·Life Sciences·Minoru MatsuiFrederick J Ehlert
Nov 24, 2004·Pharmacology & Therapeutics·Torsten SchönebergKatrin Sangkuhl
Jun 24, 2004·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Haoran WangJohn Yeomans
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Jul 2, 2009·Clinical Pharmacokinetics·Oxana Doroshyenko, Uwe Fuhr
Mar 31, 2016·Journal of Clinical Psychopharmacology·Anatoly KreininVladimir Lerner

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