PMID: 7335105Dec 1, 1981Paper

Increased efficiency of photoreversal of UV-induced dimers in the DNA of chick embryo fibroblasts with post-UV dark time

Mutation Research
W P Van de Merwe, B V Bronk

Abstract

The kinetics of photoreversal of UV-induced dimers in the DNA of early passage chick embryo fibroblasts was studied by monitoring disappearance of UV-endonuclease-sensitive sites. Photorepair was found to increase in efficiency when cells were incubated in the dark for several hours at 37 degrees C following the dimer-inducing short-wavelength (254 nm) UV treatment, but prior to the photoreactivating black light (365 nm). Following a UV dose of 10 J/m2 it took at least 4 h in the dark to saturate this effect. This UV dose inserts roughly 2.4 dimers/10(7) daltons of DNA. Dark repair removes about 0.08 dimers/h/10(7) daltons. After 6 h in the dark, exposure to black light removes an additional 1.4 dimers/10(7) daltons leaving about 0.5 dimers unaffected by this treatment. After saturation of the dark effect, the amount of photoreactivation depends only on total black light fluence and not on fluence rate for the range of rates studied. This indicates that during 30 min, the maximum time of black light exposure, no appreciable reattachment of the photorepair molecule to additional unrepaired dimer sites occurs. We estimate that the number of effective photorepair molecules per chick chick cell is at least of the order of 2 x 10(5).

References

Jan 1, 1979·Biophysical Journal·C S DownesR T Johnson
Jan 1, 1979·Annual Review of Biochemistry·P C HanawaltC A Smith
Jul 2, 1973·European Journal of Biochemistry·M Gross-BellardP Chambon
Jan 1, 1973·Photochemistry and Photobiology·R M Tyrrell
Feb 1, 1972·Experimental Cell Research·L Lima, A Macieira-Coelho
Oct 1, 1971·Analytical Biochemistry·W L Carrier, R B Setlow
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J S Cook, J R McGrath
Mar 1, 1980·Mutation Research·M H Wade, P H Lohman
Feb 1, 1965·Journal of Molecular Biology·F W STUDIER

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Citations

Apr 1, 1983·Mutation Research·B V Bronk, W P Van de Merwe
May 1, 1984·Mutation Research·B V Bronk, W P van de Merwe
Jun 8, 2002·Journal of Molecular Biology·Bernhard Suter, Fritz Thoma

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