Increased epidermal growth factor expression produced by testosterone in the submaxillary gland of female mice is accompanied by changes in poly-A tail length and periodicity

Endocrinology
L G SheflinS W Spaulding

Abstract

The level of mature EGF messenger RNA (mRNA) in the female submaxillary salivary gland (SMG) begins to rise after 3 days' treatment with testosterone (200 micrograms sc qod), and by 5 days it reaches a plateau of approximately 5 times baseline. Testosterone can increase the transcription of other genes in the SMG rapidly, so the lag in the EGF is not due to a slow androgen receptor response, and EGF mRNA can respond rapidly to other mediators, so the lag is not an innate characteristic of EGF transcription. Immunoreactive EGF levels reach a steady-state several times greater than the plateau reached by EGF mRNA, suggesting that testosterone also enhances the efficiency of EGF mRNA translation. Because testosterone has been reported to alter poly-A polymerase activities and because the translation and stability of some mRNAs is affected by changes in their polyadenylation, we used 3' rapid amplification of complementary DNA ends (3' RACE) to determine whether testosterone affected this aspect of EGF RNA metabolism. We found that EGF transcripts in untreated female SMG occur indistinct size classes, with poly-A tails of approximately 20, 50, 70, 100, and 200 A's attached after the terminal polyadenylation signal. In contrast, EGF...Continue Reading

Citations

Sep 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jean-François HuaulméIsabelle Rosinski-Chupin
Jan 26, 2005·Journal of Dental Research·N S TreisterD A Sullivan
Nov 8, 2018·Endocrine-related Cancer·Giorgio SecretoVittorio Krogh
Aug 18, 2006·Cells, Tissues, Organs·Lucila BuschEnri Borda
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Jan 25, 2000·American Journal of Physiology. Endocrinology and Metabolism·L G Sheflin, S W Spaulding
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