Increased exosome production from tumour cell cultures using the Integra CELLine Culture System

Journal of Immunological Methods
J Paul MitchellAled Clayton

Abstract

Exosomes are nanometer-sized vesicles, secreted from most cell types, with documented immune-modulatory functions. Exosomes can be purified from cultured cells but to do so effectively, requires maintenance of cells at high density in order to obtain sufficient accumulation of exosomes in the culture medium, prior to purification. Whilst high density cultures can be achieved with cells in suspension, this remains difficult with adherent cells, resulting in low quantity of exosomes for subsequent study. We have used the Integra CELLine culture system, originally designed for hybridoma cultures, to achieve a significant increase in obtainable exosomes from adherent and non-adherent tumour cells. Traditional cultures of mesothelioma cells (cultured in 75 cm(2) flasks) gave an average yield of 0.78 microg+/-0.14 microg exosome/ml of conditioned medium. The CELLine Adhere 1000 (CLAD1000) flask, housing the same cell line, increased exosome yield approximately 12 fold to 10.06 microg+/-0.97 microg/ml. The morphology, phenotype and immune function of these exosomes were compared, and found to be identical in all respects. Similarly an 8 fold increase in exosome production was obtained from NKL cells (a suspension cell line) using a CE...Continue Reading

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Citations

Mar 13, 2010·Molecular & Cellular Proteomics : MCP·Joanne L WeltonAled Clayton
Jan 14, 2009·Journal of Translational Medicine·Paul J MitchellAled Clayton
Sep 7, 2013·Journal of Extracellular Vesicles·Jason Webber, Aled Clayton
Nov 8, 2013·Functional & Integrative Genomics·Sadegh Azimzadeh JamalkandiAli Masoudi-Nejad
Feb 18, 2014·Drug Delivery and Translational Research·Keitaro HagiwaraNobuyoshi Kosaka
Feb 8, 2014·Molecular & Cellular Proteomics : MCP·Jason WebberAled Clayton
Nov 26, 2015·Cancer Letters·Radha MunagalaRamesh C Gupta
Oct 29, 2014·Molecular Psychiatry·L ChengUNKNOWN Australian Imaging, Biomarkers and Lifestyle (AIBL) Research Group
Feb 28, 2015·Seminars in Cell & Developmental Biology·David W GreeningRichard J Simpson
Apr 15, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sweta RaniThomas Ritter
Mar 31, 2015·Journal of Extracellular Vesicles·Joanne Louise WeltonAled Clayton
Apr 17, 2014·Journal of Immunological Methods·Zachary BrownleeAlan J Schroit
Oct 27, 2016·Neuroscience Letters·Yohsuke YagiTakanori Yokota
Sep 1, 2016·Journal of Extracellular Vesicles·Marie Stampe OstenfeldClaus Lindbjerg Andersen
May 13, 2017·Circulation Research·Frank A W CoumansRienk Nieuwland
Aug 1, 2018·Biotechnology and Bioengineering·Kelvin S NgJeffrey M Karp
Dec 13, 2019·Stem Cells·Yun Ha HurMarc A Antonyak
Jun 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aled ClaytonZsuzsanna Tabi
Apr 23, 2019·Journal of Extracellular Vesicles·Mari PalviainenPia R-M Siljander
Sep 27, 2020·Cytoskeleton·Sepideh ParvanianFang Cheng
Nov 26, 2010·Cancer Research·Jason WebberAled Clayton
Nov 18, 2019·Methods : a Companion to Methods in Enzymology·Melissa N Thone, Young Jik Kwon
Apr 25, 2021·Colloids and Surfaces. B, Biointerfaces·Colin L HiseyLawrence W Chamley
Mar 23, 2021·British Journal of Pharmacology·Dany PerocheauJulien Baruteau
Jun 17, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Helen L BrownPhil Stephens
Jun 21, 2021·Advanced Drug Delivery Reviews·Alice GrangierAmanda K A Silva

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