Increased expression of a disintegrin and metalloproteinase thrombospondin 1 in thrombosed hemodialysis grafts

Journal of Vascular and Interventional Radiology : JVIR
Sanjay MisraDebabrata Mukhopadhyay

Abstract

To use proteomic analysis to identify upregulated and downregulated proteins in thrombosed hemodialysis graft specimens. One of these significantly upregulated proteins was a disintegrin and metalloproteinase thrombospondin-1 (ADAMTS-1), and its expression and activity were determined in thrombosed hemodialysis grafts. Hemodialysis vascular access samples (thrombosed veins, n = 8; control veins, n = 6) were obtained from patients who required surgical revision. Proteomic analysis was performed with isotope-coded affinity tag labeling with multidimensional liquid chromatography followed by tandem mass spectrometry on four thrombosed hemodialysis graft specimens with control veins. Expression of ADAMTS-1 was confirmed by performing immunoprecipitation followed by Western blot analysis. Finally, immunohistochemistry was used to localize expression in a separate group of patients with thrombosed grafts. Thirty-nine unique proteins were common to all four patients. ADAMTS-1 was one of the only significantly upregulated protein (>38 fold). ADAMTS-1 expression was confirmed by performing immunoprecipitation and Western blot analysis and was significantly increased. ADAMTS-1 expression was localized to adventitial macrophages and neutr...Continue Reading

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Citations

Feb 28, 2013·International Journal of Cancer. Journal International Du Cancer·Izza de Arao TanDarryl L Russell
Nov 26, 2008·Journal of Vascular and Interventional Radiology : JVIR·Sanjay MisraDebabrata Mukhopadhyay
Jun 27, 2009·Journal of Vascular and Interventional Radiology : JVIR·Binxia YangSanjay Misra
Aug 12, 2008·Journal of Vascular and Interventional Radiology : JVIR·Sanjay MisraDebabrata Mukhopadhyay

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