Increased expression of colony-stimulating factor-1 in mouse spinal cord with experimental autoimmune encephalomyelitis correlates with microglial activation and neuronal loss

Glia
Svetlana GushchinaXuenong Bo

Abstract

Microglia contribute to pathophysiology at all stages of multiple sclerosis. Colony-stimulating factor-1 (CSF1) is crucial for microglial proliferation and activation. In this study we measured the CSF1 levels and studied its cellular expression in the mouse spinal cords with experimental autoimmune encephalomyelitis (EAE) to explore the potential contribution of CSF1 in neuronal death. ELISA data showed that CSF1 levels were significantly higher in the spinal cords with acute and chronic EAE than those of normal and adjuvant-injected mice. Immunohistochemical studies demonstrated that CSF1 was expressed in astrocytes and neurons in normal mouse spinal cord. In acute EAE, CSF1 expression was significantly increased, especially in astrocytes in peripheral white matter and large motoneurons. High density of activated microglia was observed in the gray matter where motoneurons expressed high-level CSF1 in acute EAE. Significant large motoneuron loss was seen in chronic EAE and the remaining motoneurons with high-level CSF1 were enwrapped by microglia. Viral vector mediated over-expression of CSF1 in spinal neurons induced profound proliferation and activation of microglia at the injection site and microglia enwrapped CSF1-transduc...Continue Reading

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Citations

Oct 25, 2020·Cell Death & Disease·Nellwyn HaganDimitry Ofengeim
Feb 24, 2021·Glia·Dave E MarzanJames L Salzer
Aug 1, 2021·Clinical and Experimental Immunology·Wissam BeainoAlbert D Windhorst
Sep 3, 2021·Neural Regeneration Research·Lorna Bo, Xuenong Bo
Sep 28, 2021·Frontiers in Immunology·John Michael S SanchezRobert S Fujinami

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