Abstract
Gangliosides GM1 is a good marker of membrane microdomains (lipid rafts) with important function in cellular activation processes. In this study we found that GM1 expression on CD4+ T cells and memory T cells (CD45RO/CD4) were dramatic increased after stimulation with phytohaemagglutinin in vitro. Next, we examined the GM1 expression on peripheral blood CD4+ T cells and CD8+ T cells from 44 patients with SLE and 28 healthy controls by flow cytometry. GM1 expression was further analyzed with serum soluble CD30 (sCD30), IL-10, TNF-alpha and clinical parameters. The mean fluorescence intensity of GM1 on CD4+ T cells from patients with SLE was significantly higher than those from healthy controls, but not on CD8+ T cells. Increased expression of GM1 was more marked on CD4+/CD45RO+ memory T cells from active SLE patients. Patients with SLE showed significantly elevated serum sCD30 and IL-10, but not TNF-alpha levels. In addition, we found that enhanced GM1 expression on CD4+ T cells from patients with SLE positively correlated with high serum levels of sCD30 and IgG as well as disease activity (SLEDAI scores). Our data suggested the potential role of aberrant lipid raft/GM1 on CD4+ T cells and sCD30 in the pathogenesis of SLE.
References
Jun 1, 1992·Arthritis and Rheumatism·C BombardierC H Chang
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Oct 20, 1999·The Journal of Cell Biology·P W JanesA I Magee
Apr 5, 2000·The Journal of Biological Chemistry·N NorazN Taylor
Nov 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Birgit KovacsTerri H Finkel
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bastian von TresckowHinrich P Hansen
Sep 24, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Robert W Hoffman
Jun 21, 2005·Molecular Immunology·Esther J PavónMercedes Zubiaur
Nov 19, 2005·Science·Kaspar D MossmanMichael L Dustin
Nov 15, 2006·Clinical Rheumatology·Hana CiferskáVlastimil Scudla
Jan 3, 2007·Nature Cell Biology·Ken JacobsonRichard G W Anderson
Feb 3, 2007·International Immunology·Lingli DongHisanori Umehara
Jun 26, 2007·Rheumatology·K TenbrockG C Tsokos
Sep 25, 2007·Seminars in Cell & Developmental Biology·Elizabeth C JuryPanagiotis S Kabouridis
Oct 4, 2007·Journal of Clinical Immunology·Nima RezaeiZahra Pourpak
Oct 17, 2007·Clinical and Experimental Immunology·L DongH Umehara
Sep 30, 2008·International Immunology·Zhe-Xiong JinHisanori Umehara
Oct 22, 2008·FEBS Letters·Panagiotis S Kabouridis, Elizabeth C Jury
Feb 14, 2009·Lupus·A La Cava
Feb 28, 2009·Arthritis and Rheumatism·Jinoos YazdanyUNKNOWN Systemic Lupus Erythematosus Quality Indicators Project Expert Panels
May 13, 2009·Lupus·S DolffC G M Kallenberg
Jul 30, 2009·Blood·Mark P PurdueNathaniel Rothman
Sep 22, 2009·Transplantation Proceedings·G SolgiB Nikbin
Citations
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Masakazu NagafukuJin-ichi Inokuchi
Mar 20, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Joseph M AhearnSusan Manzi
Dec 16, 2011·Trends in Immunology·Michael CroftAmit Mehta
Aug 15, 2015·BioMed Research International·Rongfen GaoLingli Dong
Aug 2, 2013·Therapeutic Advances in Musculoskeletal Disease·Chau-Ching LiuJoseph M Ahearn
Oct 16, 2018·FEBS Letters·Hitoshi NakayamaJin-Ichi Inokuchi
Mar 19, 2020·PloS One·Tamara K NowlingGary Gilkeson
Dec 6, 2019·Eye·Ahmed M Abu El-AsrarSofie Struyf
Dec 23, 2020·International Journal of Molecular Sciences·Berkan SavasMario Ollero
Apr 1, 2021·Kidney International·Katharina ArtingerKathrin Eller