Increased expression of glycosyl-phosphatidylinositol anchor attachment protein 1 (GPAA1) is associated with gene amplification in hepatocellular carcinoma

International Journal of Cancer. Journal International Du Cancer
Jenny C HoSheung Tat Fan

Abstract

Glycosyl-phosphatidylinositol (GPI) anchor attachment protein 1 (GPAA1) transcript level was frequently up-regulated in our earlier study on gene expression profile. We therefore analyzed the potential involvement of GPAA1 in hepatocellular carcinoma (HCC) as GPAA1 gene locates at chromosome 8q24.3 which chromosome region is frequently amplified in HCCs. In this study, we observed that GPAA1 transcript in the HCCs (n = 93) showed a significantly higher expression level compared with their paralleled adjacent nontumor liver tissues, cirrhosis (n = 15) and normal (n = 16) liver tissues using real-time quantitative RT-PCR (p < 0.005). We also demonstrated that GPAA1 protein up-regulation was common in HCCs (90%, 9/10), and GPAA1 gene was frequently amplified (73%, 11/15) using quantitative microsatellite analysis. Increased GPAA1 expression was significantly associated with HCCs poor cellular differentiation (p = 0.011) and poor prognosis (p = 0.010). We then modulated the GPAA1 expression level in HCC cells (Hep3B) by transfection experiments, which was shown to positively regulate cell adhesion ability (p = 0.004) and proliferation rate (p = 0.037). Our data revealed GPAA1 gene amplification with overexpression of RNA and protei...Continue Reading

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Citations

May 15, 2007·Journal of Cancer Research and Clinical Oncology·Jenny C Y HoSheung Tat Fan
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Apr 9, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jenny C HoSheung Tat Fan
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May 24, 2019·Journal of Experimental & Clinical Cancer Research : CR·Xiao-Xin ZhangZhi-Gang Zhang
May 29, 2020·BioMed Research International·Yali GaoBing Song

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