Increased expression of H+-ATPase in inner medullary collecting duct of aquaporin-1-deficient mice

American Journal of Physiology. Renal Physiology
Young-Hee KimKirsten M Madsen

Abstract

Phenotype analysis has demonstrated that aquaporin-1 (AQP1) null mice are polyuric and manifest a urinary concentrating defect because of an inability to create a hypertonic medullary interstitium. We report here that deletion of AQP1 is also associated with a decrease in urinary pH from 6.15 +/- (SE) 0.1 to 5.63 +/- 0.07. To explore the mechanism of the decrease in urinary pH, we examined the expression of H+-ATPase in kidneys of AQP1 null mice. There was strong labeling for H+-ATPase in intercalated cells and proximal tubule cells in both AQP1 null and wild-type mice. Strong H+-ATPase immunostaining was also present in the apical plasma membrane of inner medullary collecting duct (IMCD) cells in AQP1 null mice, whereas no H+-ATPase labeling was observed in IMCD cells in wild-type mice. In addition, there was an increase in the prevalence of type A intercalated cells in the IMCD of AQP1 null mice, suggesting that the deletion of intercalated cells from the IMCD, which normally occurs during postnatal kidney development, was impaired. Western blot analysis of H+-ATPase expression in the different regions of the kidney demonstrated a significant increase in H+-ATPase protein in the inner medulla of AQP1 null mice compared with w...Continue Reading

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Citations

Oct 27, 2009·The Journal of Clinical Investigation·Hyun-Woo JeongYoung-Yun Kong
Oct 31, 2006·Seminars in Nephrology·Juan Codina, Thomas D DuBose
Sep 24, 2004·Physiological Reviews·Carsten A WagnerJohn P Geibel
May 16, 2006·American Journal of Nephrology·Hassane AmlalManoocher Soleimani
Apr 27, 2019·Bioscience Reports·Ying HuaXueqiong Zhu
Feb 24, 2012·American Journal of Physiology. Cell Physiology·Helle Hasager Damkier, Jeppe Praetorius

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