PMID: 16538044Mar 16, 2006Paper

Increased expression of heat shock protein (HSP)72 in a human proximal tubular cell line (HK-2) with gentamicin-induced injury

The Journal of Toxicological Sciences
Wang ZhipengZhang Rong

Abstract

Gentamicin (GM) has been widely used as an antibiotic and its nephrotoxicity has been recognized. However, the alternation of heat shock protein (HSP) 72 as an inductive protein in proximal tubular cells treated with GM is still unclear. In this study, GM cytotoxicity and its effect on the expression of HSP72 in human kidney proximal tubular (HK-2) cells were measured. HK-2 cells were incubated for 24 hr, 48 hr, 72 hr, and 96 hr with GM only and GM plus MnCl2, respectively. Cytotoxicity was determined by the release of lactate dehydrogenase (LDH). Activity of N-acetyl-beta-D-glucosaminidase (NAG) and effects of GM on oxidation in HK-2 cells were investigated by measurements of malondialdehyde (MDA) content and superoxide dismutase (SOD) activity, and the ability of viable cells to reduce a tetrazolium-based compound (MTT). The expression of HSP72 was measured by immunocytochemistry, Western blotting and RT-PCR. Cells were exposed to GM at a concentration of 100 microg/ml. After 24 hr MTT uptake decreased significantly and then gradually until 96 hr. LDH release increased time-dependently from 24 hr to 72 hr, but decreased at 96 hr compared with the data at 72 hr when cells were treated with GM only. Both results of NAG and SOD ...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M A BrownJ L Renfro
Jan 1, 1991·The International Journal of Biochemistry·H Itoh, Y Tashima
Dec 15, 1990·Biochemical Pharmacology·Y R DonatiB S Polla
Jan 1, 1990·Methods in Enzymology·H H Draper, M Hadley
Dec 1, 1987·Annals of Internal Medicine·J M EisenbergB L Strom
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K Y Hostetler, L B Hall
Sep 1, 1982·Antimicrobial Agents and Chemotherapy·W M BennettD A McCarron
Mar 10, 1995·The Journal of Biological Chemistry·C Y LiJ B Little
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·B S PollaA Cossarizza
Jun 26, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·A KomatsudaY Tashima
Jul 23, 1999·Renal Failure·P D WalkerS V Shah
Nov 27, 1999·Drug Metabolism Reviews·R BaligaS V Shah
Nov 7, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B H AliH M Mousa
Nov 22, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tomoko MatsushitaMakoto Kurokawa
Nov 30, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Elisabetta CasalinoClemente Landriscina
Feb 18, 2004·The Journal of Biological Chemistry·Toshio MiyazakiHideaki Itoh
May 20, 2004·Kidney International·Carlos Martínez-SalgadoJosé M López-Novoa

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Citations

Jan 5, 2011·EMBO Molecular Medicine·Jonatan Barrera-ChimalNorma A Bobadilla
Jan 27, 2015·International Journal of Molecular Sciences·Michela CodiniElisabetta Albi
Nov 11, 2014·Expert Opinion on Drug Metabolism & Toxicology·Johnny X HuangMatthew A Cooper
Jul 1, 2008·Environmental Toxicology and Pharmacology·Sivakumar J T Gowder, Halagowder Devaraj
Aug 12, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Beom Jin LimHyeon Joo Jeong
Mar 31, 2015·Indian Journal of Pharmacology·Abdulkadir A Alnakshbandi
Apr 15, 2017·Cell Stress & Chaperones·Natalia ChebotarevaEvgeniy Shilov
Dec 29, 2007·Critical Reviews in Toxicology·Helmut H ZepikDavid M Yourtee

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