Increased expression of integrin-linked kinase improves cardiac function and decreases mortality in dilated cardiomyopathy model of rats.

PloS One
Rong GuBiao Xu

Abstract

Integrin-linked kinase (ILK) is a multifunctional kinase linking the extracellular matrix to intracellular signaling pathways, whose activation in the heart gives rise to a number of functional consequences. The aim of this study is to demonstrate the therapeutic and survival benefit of cardiac ILK overexpression in a rat model of dilated cardiomyopathy. The dilated cardiomyopathy model was generated in rats by intraperitoneal administration of six equal doses of doxorubicin over a 2 week period. Five weeks after the first injection, echocardiographic analysis demonstrated impaired cardiac function and, at that point, recombinant adenoviral vector harboring ILK cDNA or vehicle was injected into the myocardium, and the rats re-studied 4 weeks later. Compared with vehicle injection, ILK treatment ameliorated inflammatory cell infiltration and cardiomyocyte degeneration, as well as left ventricular dilation and dysfunction. ILK treatment was also associated with a reduction in apoptosis and an increase in proliferation of cardiomyocytes, as well as decreased oxidative stress and autophagic vacuole accumulation. Importantly, mortality was lower in rats following ILK treatment than in those following vehicle injection. In cultured n...Continue Reading

References

Sep 24, 1998·The New England Journal of Medicine·P K Singal, N Iliskovic
Dec 2, 2000·Science·D J Klionsky, S D Emr
Dec 31, 2002·Journal of Molecular and Cellular Cardiology·Semon WuYing-Shiung Lee
Apr 6, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Michiko IshidaSoichiro Kitamura
May 12, 2004·Journal of Molecular and Cellular Cardiology·H LouP K Singal
Jan 5, 2005·Nature Reviews. Cancer·Gregory HanniganShoukat Dedhar
Dec 5, 2006·The Journal of Thoracic and Cardiovascular Surgery·Hideaki YamabiJohn G Coles
Feb 3, 2007·Nature Reviews. Drug Discovery·David M Kaye, Henry Krum
Mar 6, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Longhu LiHisayoshi Fujiwara
Mar 31, 2007·Journal of Cardiac Failure·Ioannis ParaskevaidisDimitrios Th Kremastinos
Nov 22, 2007·Trends in Molecular Medicine·Wim MartinetGuido R Y De Meyer

❮ Previous
Next ❯

Citations

Mar 22, 2014·Nature Reviews. Molecular Cell Biology·Sabina E Winograd-KatzKyle R Legate
Oct 13, 2014·International Journal of Cardiology·Santiago RouraAntoni Bayes-Genis
Oct 8, 2014·Chest·Dragana NikitovicGeorge N Tzanakakis
Oct 11, 2012·Annals of the New York Academy of Sciences·Sudhiranjan GuptaIl-Kwon Kim
Sep 4, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·De-Lin WangWei-Xue Tang
Jan 21, 2015·Cardiovascular Research·Dirk J DunckerLucie Carrier
Dec 7, 2016·Journal of Zhejiang University. Science. B·Li-Juan ShenYong-Liang Xu
Sep 24, 2014·Molecular and Cellular Biology·Marie-Claude PerryVincent Giguère
Jun 14, 2015·Circulation. Arrhythmia and Electrophysiology·Khai Le QuangStanley Nattel
Jul 20, 2019·Bioscience Reports·Xiaonan ZhangJunping Zhang
Mar 9, 2021·Technology and Health Care : Official Journal of the European Society for Engineering and Medicine·Yaling LiJun Yang
Oct 28, 2017·European Journal of Pharmacology·Kaviyarasi RenuSankarganesh Arunachalam

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
electrophoresis
ELISA
MDA
transmission electron microscopy
PCR
transfection
flow cytometry

Software Mentioned

Image Pro Plus

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Cardiac Remodeling

Cardiac remodeling in response to a myocardial infarction is characterized by progressive ventricular dilatation, cardiac hypertrophy, fibrosis, and deterioration of cardiac performance. Discover the latest research on Cardiac Remodeling here.

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.