Increased expression of native cytosolic Cu/Zn superoxide dismutase and ascorbate peroxidase improves tolerance to oxidative and chilling stresses in cassava (Manihot esculenta Crantz)

BMC Plant Biology
Jia XuPeng Zhang

Abstract

Cassava (Manihot esculenta Crantz) is a tropical root crop, and is therefore, extremely sensitive to low temperature; its antioxidative response is pivotal for its survival under stress. Timely turnover of reactive oxygen species (ROS) in plant cells generated by chilling-induced oxidative damages, and scavenging can be achieved by non-enzymatic and enzymatic reactions in order to maintain ROS homeostasis. Transgenic cassava plants that co-express cytosolic superoxide dismutase (SOD), MeCu/ZnSOD, and ascorbate peroxidase (APX), MeAPX2, were produced and tested for tolerance against oxidative and chilling stresses. The up-regulation of MeCu/ZnSOD and MeAPX2 expression was confirmed by the quantitative reverse transcriptase-polymerase chain reaction, and enzymatic activity analyses in the leaves of transgenic cassava plant lines with a single-transgene integration site. Upon exposure to ROS-generating agents, 100 μM ROS-generating reagent methyl viologen and 0.5 M H₂O₂, higher levels of enzymatic activities of SOD and APX were detected in transgenic plants than the wild type. Consequently, the oxidative stress parameters, such as lipid peroxidation, chlorophyll degradation and H₂O₂ synthesis, were lower in the transgenic lines th...Continue Reading

References

Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Apr 1, 1968·Archives of Biochemistry and Biophysics·R L Heath, L Packer
Apr 29, 1998·Plant Physiology· del Rio LAJ A Hernandez
Feb 24, 2001·Transgenic Research·P ZhangJ Puonti-Kaerlas
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 9, 2002·Journal of Experimental Botany·Shigeru ShigeokaKazuya Yoshimura
May 9, 2002·Journal of Experimental Botany·Ruth Grene AlscherLenwood S Heath
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·Yukinori YabutaShigeru Shigeoka
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Jan 5, 2005·Plant Molecular Biology·James V AndersonDavid P Horvath
Jan 5, 2005·Plant Molecular Biology·Camilo LopezValérie Verdier
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D A DaltonH J Evans
Nov 4, 2006·Plant, Cell & Environment·Petra ZimmermannUlrike Zentgraf
Mar 19, 2008·Physiologia Plantarum·Gad MillerRon Mittler
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
May 30, 2013·Frontiers in Physiology·Emmanuel OkogbeninMartin Fregene
Jul 1, 2009·Comprehensive Reviews in Food Science and Food Safety·Julie A MontagnacSherry A Tanumihardjo

❮ Previous
Next ❯

Citations

Jan 23, 2016·Acta Biochimica Et Biophysica Sinica·Pingan HanXiaoyu Zhang
Nov 20, 2016·Plant Biotechnology Journal·Weijuan FanPeng Zhang
Nov 25, 2016·Plant Molecular Biology Reporter·Feifei AnSongbi Chen
Jun 10, 2015·Functional & Integrative Genomics·Xiangpeng LengJinggui Fang
Nov 9, 2015·Journal of Plant Research·Maliwan NaconsieJarunya Narangajavana
Dec 18, 2019·Journal of Food Biochemistry·Duvvarapu Easwar Rao, Kolluru Viswanatha Chaitanya
Nov 19, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Shashi ShekharKapil Lawrence
May 6, 2017·Frontiers in Plant Science·Saurabh PandeyMalireddy K Reddy
Sep 1, 2017·Frontiers in Plant Science·Ali NomanShuilin He
Jul 4, 2018·Frontiers in Plant Science·Lixia HouXin Liu
Nov 13, 2019·Scientific Reports·Prashanti PatelThumballi Ramabhatta Ganapathi
Jun 18, 2019·BioMed Research International·Xiulan XieYi Cai
Mar 22, 2021·Journal of Plant Physiology·Wenqiang WangQunqun Hao
Aug 28, 2021·International Journal of Molecular Sciences·Xuan Lan Thi HoangNguyen Phuong Thao

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
environmental stress
environmental stresses
phosphotransferase
MDA
PCR
electrophoresis

Software Mentioned

SPSS
Primer 3 Plus

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.