Increased expression of toll-like receptor 2 in rat diabetic nephropathy

American Journal of Nephrology
Fanglin LiXueqing Yu

Abstract

Inflammation is implicated in the pathogenesis of diabetic nephropathy (DN). This study examined the role of Toll-like receptor 2 (TLR2) in the progression of renal injury in a model of rat DN. DN was induced by intravenous injection of streptozotocin and rats were sacrificed at week 2, 4 and 8. Functional and pathologic markers, inflammatory infiltration, expression of TLR2, MCP-1, MyD88, HSP70, HMGB1 and activation of NF-kappaB were assessed. The effects of glucose on the expression of TLR2 by renal tubular epithelial cells were also examined in vitro. The expression of TLR2 mRNA and protein level was significantly upregulated in the kidneys of diabetic rats (p < 0.01), which was associated with increased renal expression of MyD88 and MCP-1, activation of NF-kappaB and infiltration of macrophages. The expression of HSP70 and HMGB1, endogenous ligands of TLRs, was also significantly upregulated in the kidneys of diabetic rats. In human renal biopsy of DN, there was prominent expression of TLR2 in both the glomeruli and tubulointerstitium. In vitro study showed that high glucose induced the expression of TLR2 mRNA by NRK-52E cells (p < 0.01). Enhanced renal expression of TLR2 is associated with inflammatory infiltration in DN.

Citations

Jun 27, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Sydney C W Tang, Kar Neng Lai
Aug 10, 2012·American Journal of Physiology. Renal Physiology·Harmeet KaurIshwarlal Jialal
May 28, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Sridevi DevarajIshwarlal Jialal
Dec 14, 2011·PloS One·Billy D NovosadMichelle C Callegan
Oct 25, 2011·Journal of the American Society of Nephrology : JASN·Miao LinSydney C W Tang
Nov 10, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Miao Lin, Sydney C W Tang
Nov 16, 2013·Archives of Medical Research·Paul Leurs, Bengt Lindholm
Aug 28, 2015·Kidney International Supplements·Wai Han YiuSydney C W Tang
Jan 13, 2015·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Sydney C W TangKar Neng Lai
Oct 19, 2012·Clinical Science·Jun Wada, Hirofumi Makino
Jul 11, 2014·Molecular Aspects of Medicine·Rui KangDaolin Tang
Feb 5, 2014·Clinical Science·Harshini MudaliarUsha Panchapakesan
Nov 26, 2015·Science Advances·Yuanyuan FengXiangmei Chen
Oct 12, 2017·Diabetology & Metabolic Syndrome·Koichiro KajiwaraYoshihiko Sawa
Apr 12, 2013·American Journal of Physiology. Renal Physiology·Harshini MudaliarUsha Panchapakesan
Nov 17, 2016·Mediators of Inflammation·Han WuBiao Xu
Aug 2, 2017·Clinical Science·Greg H Tesch
Sep 17, 2020·International Journal of Molecular Sciences·Sebastian MertowskiWojciech Załuska
Aug 22, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Kouhei OhnoTetsuji Miura
Mar 30, 2017·Experimental and Therapeutic Medicine·Fanglin LiYunfeng Zhou
Oct 24, 2017·Current Opinion in Nephrology and Hypertension·Usha Panchapakesan, Carol Pollock

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