Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes

Archives of Biochemistry and Biophysics
M Clara De PascaleJean-Marc Zingg

Abstract

In skin, vitamin E acts as the predominant lipophilic antioxidant with a protective function against irradiation and oxidative stress. In addition to that, vitamin E can also modulate signal transduction and gene expression. To study whether the four natural tocopherol analogues (alpha-, beta-, gamma-, delta-tocopherol) can influence transcriptional activity by modulating the activity of nuclear receptors, a human keratinocytes cell line (NCTC 2544) was transfected with plasmids containing the luciferase reporter gene under control by direct repeat elements (DR1-DR4), representing binding sites for four different classes of nuclear receptors. In this model, the tocopherols positively modulated only the reporter construct containing a consensus element for peroxisome proliferator-activated receptors (PPARs). The induction was strongest with gamma-tocopherol and was most likely the direct consequence of stimulation of PPARgamma protein expression in keratinocytes. Vitamin E treatment also led to increased expression of a known PPARgamma target gene involved in terminal keratinocytes differentiation, the transglutaminase-1.

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Citations

Sep 15, 2009·Carcinogenesis·Jihyeung JuChung S Yang
Nov 1, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J G WagnerD B Peden
Jul 18, 2015·Annual Review of Nutrition·Jean-Marc Zingg
Sep 3, 2016·Poultry Science·Tamara KorošecJanez Salobir
Feb 24, 2007·Molecular Aspects of Medicine·Elke ReiterStephan Christen
Jul 13, 2007·Molecular Aspects of Medicine·Luis VillacortaJean-Marc Zingg
Feb 7, 2018·Journal of Nutrigenetics and Nutrigenomics·Wendy Campos-PerezErika Martinez-Lopez
Dec 18, 2018·IUBMB Life·Jean-Marc Zingg
Dec 1, 2006·Nutrition Research Reviews·Regina Brigelius-Flohé
May 20, 2008·Free Radical Research·Jean-Francois LandrierPatrick Borel
Aug 16, 2014·International Wound Journal·Rachel Hobson
Sep 13, 2019·Journal of Diabetes Research·Olvera-Montaño CeciliaRodríguez-Carrizalez Adolfo Daniel
Jun 15, 2007·Free Radical Biology & Medicine·Maret G Traber, Jeffrey Atkinson
Jan 10, 2009·Free Radical Biology & Medicine·Regina Brigelius-Flohé
Oct 19, 2020·Dermatology : International Journal for Clinical and Investigative Dermatology·Cheryl Wei Ling TeoWei Ney Yap

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