Increased expression of upstream TH2-cytokines in a mouse model of viral-induced asthma exacerbation

Journal of Translational Medicine
Irma Mahmutovic PerssonLena Uller

Abstract

Exacerbations of asthma caused by respiratory viral infections are serious conditions in need of novel treatment. To this end animal models of asthma exacerbations are warranted. We have shown that dsRNA challenges or rhinoviral infection produce exacerbation effects in mice with ovalbumin (OVA)-induced allergic asthma. However, house dust mite (HDM) is a more human asthma-relevant allergen than OVA. We thus hypothesised that dsRNA challenges in mice with HDM-induced experimental asthma would produce important translational features of asthma exacerbations. Mouse airways were challenged locally with HDM or saline three times a week for three weeks to establish experimental asthma. Then daily local dsRNA challenges were given for three consecutive days to induce exacerbation. Bronchoalveolar lavage fluid (BALF) was analysed for inflammatory cells, total protein, the necrosis marker LDH and the alarmin ATP. Lung homogenates were analysed for mRNA expression (RT-qPCR) of TNF-α, CCL2, CCL5, IL-1β, IL-33, thymic stromal lymphopoietin (TSLP), and IL-25 as well as pattern recognition receptors (PRRs) RIG-I, MDA5 and TLR3. Lung tissue IL-33 was analysed with ELISA and PRRs were quantified by western blot. Immunohistochemistry indicated...Continue Reading

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Citations

May 19, 2018·The European Respiratory Journal·Philippe BonniaudMartin Kolb
Mar 11, 2018·Scientific Reports·Samuel C CerpsLena Uller
Mar 21, 2019·Allergy·Kazuhiko MaedaShizuo Akira
Dec 7, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S RamuL Uller

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Methods Mentioned

BETA
bronchoalveolar lavage
dissection
ELISA
Protein Assay
PCR

Software Mentioned

Excel
ImageStudio
Aperio ScanScopeTM
GraphPad Prism
PrimerDesign

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