Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles

Materials
Jeimmy González-MasísRodolfo J Gonzalez-Paz

Abstract

Propolis natural extracts have been used since ancient times due to their antioxidant, anti-inflammatory, antiviral, and antimicrobial activities. In this study, we produced scaffolds of type I collagen, extracted from Wistar Hanover rat tail tendons, and impregnated them with propolis nanoparticles (NPs) for applications in regenerative medicine. Our results show that the impregnation of propolis NPs to collagen scaffolds affected the collagen denaturation temperature and tensile strength. The changes in structural collagen self-assembly due to contact with organic nanoparticles were shown for the first time. The fibril collagen secondary structure was preserved, and the D-pattern gap increased to 135 ± 28 nm, without losing the microfiber structure. We also show that the properties of the collagen scaffolds depended on the concentration of propolis NPs. A concentration of 100 μg/mL of propolis NPs with 1 mg of collagen, with a hydrodynamic diameter of 173 nm, was found to be an optimal concentration to enhance 3T3 fibroblast cell metabolic activity and cell proliferation. The expected outcome from this research is both scientifically and socially relevant since the home scaffold using natural nanoparticles can be produced usi...Continue Reading

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Citations

Feb 13, 2021·International Journal of Molecular Sciences·Luis Castillo-HenríquezJosé Vega-Baudrit
Jan 21, 2021·Biomimetics·Martina Rossi, Pasquale Marrazzo
Aug 29, 2021·Plants·Maria Jesús Lisbona-GonzálezJorge Moreno-Fernandez

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Methods Mentioned

BETA
dynamic
atomic
differential scanning calorimetry
infrared spectroscopy
atomic force microscopy
AFM

Software Mentioned

Nanoscope
RStudio

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