Increased formation of phosphatidylinositol-4-phosphate in human platelets stimulated with lysophosphatidic acid

Lipids
I ManiB J Holub

Abstract

Lysophosphatidic acid (LPA, 1-acyl-sn-glycerol 3-phosphate), at a concentration of 1-40 microM, was found to induce the formation of [3H]inositol-labelled phosphatidylinositol-4-phosphate (PIP) without significantly altering the levels of either phosphatidylinositol (PI) or phosphatidylinositol bisphosphate (PIP2) in washed human platelets. Preincubation of platelets with the cyclooxygenase/lipoxygenase inhibitor, BW755C at 100 microM, did not alter the LPA-induced formation of PIP. Activation of platelets with the phorbol ester, phorbol 12-myristate 13-acetate (PMA), elicited a similar response (induction of PIP formation). The specific protein kinase C (PKC) inhibitor, GF109203X (10 microM), completely blocked the effect of PMA but not the LPA-induced generation of PIP. The present results indicate that LPA can induce PIP formation via PI-4-kinase activation, through processes which are independent of the eicosanoid/TxA2 pathway and are not PKC-dependent.

References

Nov 1, 1978·Prostaglandins and Medicine·J M GerrardJ G White
Jan 1, 1992·Progress in Lipid Research·L M Thomas, B J Holub
Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·W H MoolenaarW J van Blitterswijk
Jul 16, 1990·Biochemical and Biophysical Research Communications·D C Gaudette, B J Holub
Sep 17, 1984·Biochemical and Biophysical Research Communications·D de Chaffoy de CourcellesH Van Belle
Oct 4, 1984·Biochimica Et Biophysica Acta·J M Gerrard, P Robinson
Mar 31, 1981·Biochemical and Biophysical Research Communications·A TokumuraH Tsukatani
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