Increased gamma-tubulin expression and P16INK4A promoter methylation occur together in preinvasive lesions and carcinomas of the breast

Annals of Oncology : Official Journal of the European Society for Medical Oncology
Tieju LiuFei Zhang

Abstract

Loss of p16(INK4A) due to promoter hypermethylation is correlated with the ability to acquire centrosomal abnormalities in variant human mammary epithelial cells. gamma-Tubulin is a highly conserved component of centrosome in most animal cells and gamma-tubulin protein overexpression could lead to centrosome aberration. A large series of breast premalignant lesions and carcinoma was analyzed. Real-time quantitative PCR and immunohistochemistry were carried out to measure gamma-tubulin copy numbers and protein expression. MethyLight and immunohistochemistry were carried out to determine p16(INK4A) methylation and protein expression. gamma-Tubulin protein expression was concordant with gene amplification; both of them were found to increase with atypical ductal hyperplasia-carcinoma sequence. The median value and positive rate of p16(INK4a) methylation increased while protein expression displayed a decreasing trend. P16(INK4a) methylation showed a firm association with gamma-tubulin gene amplification. gamma-Tubulin gene amplification and the concomitant protein overexpression present not only in invasive carcinoma but also in a significant fraction of atypical hyperplasia and in situ carcinomas. P16(INK4a) methylation and gamma-...Continue Reading

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Citations

Aug 18, 2010·Journal of Experimental & Clinical Cancer Research : CR·Xiao-yun MaoFeng Jin
Nov 2, 2011·International Journal of Biological Sciences·Jason Yongsheng Chan
Aug 15, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chuifeng FanEnhua Wang
Mar 14, 2014·Neurological Research·Hung-Pei TsaiJoon-Khim Loh
Oct 21, 2015·Analytical Cellular Pathology (Amsterdam)·Daniele FanaleViviana Bazan
Aug 8, 2006·Current Biology : CB·Manjari MazumdarTom Misteli
Sep 13, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiao-Yun MaoFeng Jin
Jan 25, 2012·Chemical Biology & Drug Design·Douglas E FriesenJack A Tuszynski
Aug 4, 2009·Journal of Cellular Physiology·Christos D KatsetosPavel Dráber
Sep 16, 2017·Breast Cancer Research and Treatment·David N Danforth

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