PMID: 11337363May 5, 2001Paper

Increased glomerular and tubular expression of transforming growth factor-beta1, its type II receptor, and activation of the Smad signaling pathway in the db/db mouse

The American Journal of Pathology
S W HongF N Ziyadeh

Abstract

Activation of the renal transforming growth factor-beta (TGF-beta) system likely mediates the excess production of extracellular matrix in the diabetic kidney. To establish the role of the TGF-beta system in type 2 diabetic nephropathy, we examined the intrarenal localization and expression of the TGF-beta1 isoform, the TGF-beta type II receptor, and the Smad signaling pathway in the 16-week-old db/db mouse, a genetic model of type 2 diabetes that exhibits mesangial matrix expansion, glomerular basement membrane thickening, and renal insufficiency that closely resemble the human disease. Compared with its nondiabetic db/m littermate, the db/db mouse showed significantly increased TGF-beta1 mRNA expression by in situ hybridization in both glomerular and tubular compartments. Likewise, TGF-beta1 protein, by immunohistochemical staining, was increased in both renal compartments, but the fractional expression of TGF-beta1 protein was less than that of the mRNA in the glomerulus. In situ hybridization and immunohistochemical staining for the TGF-beta type II receptor revealed concordant and significant increases of both mRNA and protein in the glomerular and tubular compartments of diabetic animals. Finally, immunohistochemistry sho...Continue Reading

References

Feb 3, 1992·Biochimica Et Biophysica Acta·B L Blazer-YostF N Ziyadeh
Dec 1, 1980·Experimental and Molecular Pathology·S M Lee, A Graham
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T YamamotoW A Border
Aug 1, 1994·Kidney International·S J ShanklandK Thai
Jun 25, 1998·Experimental Nephrology·M P CohenF N Ziyadeh
Dec 29, 1998·Cell·R DerynckX H Feng
Mar 4, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D KoyaR Kikkawa
Apr 25, 2000·The EMBO Journal·J Massagué, D Wotton
Nov 30, 2000·Journal of the American Society of Nephrology : JASN·Motohide IsonoFuad N Ziyadeh

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Citations

Nov 13, 2003·Current Diabetes Reports·Gunter Wolf
Sep 5, 2002·Biochemical and Biophysical Research Communications·Motohide IsonoFuad N Ziyadeh
Oct 9, 2003·Kidney International·Ji Hoon KimHyun Soon Lee
May 23, 2013·Omics : a Journal of Integrative Biology·Vinay RandhawaGanesh Bagler
Sep 11, 2010·Investigative Radiology·Pottumarthi PrasadDaniel Batlle
Dec 22, 2004·Kidney International·Rangnath MishraMichael S Simonson
Apr 9, 2010·American Journal of Physiology. Renal Physiology·Youli WangCharles W Heilig
Sep 24, 2010·Fibrogenesis & Tissue Repair·Rainer GirgertOliver Gross
Mar 5, 2011·Journal of the American Society of Nephrology : JASN·Ivonne LoefflerGunter Wolf
Nov 5, 2011·Journal of the American Society of Nephrology : JASN·Gwon-Soo JungIn-Kyu Lee
Feb 22, 2005·Redox Report : Communications in Free Radical Research·Yuji NaitoToshikazu Yoshikawa
Jun 6, 2013·TheScientificWorldJournal·Masako FurukawaYasuhiko Tomino
Jun 3, 2014·BMC Complementary and Alternative Medicine·Yanna NieQian Wang
Apr 10, 2014·Journal of Traditional and Complementary Medicine·Thing-Fong TzengI-Min Liu
Dec 12, 2012·The International Journal of Biochemistry & Cell Biology·Rojeen ShahniAfshan N Malik
Jan 14, 2016·International Urology and Nephrology·Ionel Alexandru ChecheriţăAdrian Covic
Nov 25, 2006·Journal of Ethnopharmacology·Takako NakagawaYutaka Shimada
May 28, 2003·Biochemical and Biophysical Research Communications·Masaki FujimotoSeijiro Mori

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