Increased glycosylation of serum human chorionic gonadotropin and subunits from eutopic and ectopic sources: comparison with placental and urinary forms

The Journal of Clinical Endocrinology and Metabolism
H G FeinB D Weintraub

Abstract

The role of carbohydrate in the heterogeneity of hCG and its subunits is unclear. To study this question, we chromatographed over Sephadex G-100 an extract of term placenta as well as sera from a woman in the first and third trimesters of pregnancy and sera from two patients with nontrophoblastic malignancies. Samples were cochromatographed with radiolabeled urinary standards. hCG from first trimester pregnancy serum showed multiple peaks on G-100. The dominant peak eluted with an apparent molecular weight (72,000) higher than that of hCG from third trimester serum (63,000), urine (6),000), and placenta (59,000). hCG from both malignancy sera eluted with an apparent molecular weight (62,000) similar to that of hCG from third trimester and urinary hCG. Free hCG alpha from all sera eluted with a similar apparent molecular weight (29,000), which was higher than that of placental and urinary free alpha-subunit (22,000) and the alpha-subunit dissociated from intact hCG from all sources (22,000--23,000). The subunits were dissociated in the denaturing medium of 6 M guanidine-HCl, pH 3.0, and chromatographed in this medium over Sepharose CL-6B. This eliminated all of the differences in apparent molecular weight among corresponding for...Continue Reading

Citations

Jan 13, 1999·Prenatal Diagnosis·J F O'ConnorG Kovalevskaya
Jan 1, 1991·Prenatal Diagnosis·P G KratzerR N Taylor
Sep 21, 2002·Molecular and Cellular Endocrinology·G KovalevskayaJ F O'Connor
Dec 20, 1996·Molecular and Cellular Endocrinology·H Alfthan, U H Stenman
Oct 24, 2008·Human Reproduction Update·S F de Medeiros, R J Norman
Sep 13, 2005·Journal of Immunoassay & Immunochemistry·Pramod V PrasadK Muralidhar
Jan 1, 1987·Virchows Archiv. A, Pathological Anatomy and Histopathology·M FukayamaM Koike
Aug 15, 1984·Archives of Biochemistry and Biophysics·D S McClure, G S Cox
Apr 15, 1983·Biochemical and Biophysical Research Communications·J S MorrowS W Rosen

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