Abstract
To investigate the ultra-structural changes and heat shock protein 70 (HSP70) expression in the pancreas of rats with endotoxic shock and to detect their possible relationship. A total of 33 Wistar rats were randomly divided into three groups: control group (given normal saline), small dose lipopolysaccharide (LPS) group (given LPS 5 mg/kg) and large dose LPS group (given LPS 10 mg/kg). Pancreas was explanted to detect the ultra-structural changes by TEM and the HSP70 expression by immunohistochemistry and Western blot. Rats given small doses of LPS showed swelling and loss of mitochondrial cristae of acinar cells and increased number of autophagic vacuoles in the cytoplasm of acinar cells. Rats given large doses of LPS showed swelling, vacuolization, and obvious myeloid changes of mitochondrial cristae of acinar cells, increased number of autophagic vacuoles in the cytoplasm of acinar cells. HSP70 expression was increased compared to the control group (P<0.05). Small doses of LPS may induce stronger expression of HSP70, promote autophagocytosis and ameliorate ultra-structural injuries.
References
Jan 1, 1988·Annual Review of Genetics·S Lindquist, E A Craig
Apr 1, 1989·Archives of Surgery·W J SchirmerD E Fry
Jan 1, 1993·The Journal of Experimental Medicine·M Jäättelä, D Wissing
Mar 27, 1997·The New England Journal of Medicine·G R BernardB B Swindell
Mar 19, 1999·Acta Anaesthesiologica Scandinavica·K A Kirkebøen, O A Strand
Mar 18, 2000·Scandinavian Journal of Immunology·P M WongB M Sultzer
Apr 7, 2000·The Journal of Biological Chemistry·V BurkartH Kolb
Apr 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·S S LauR Mestril
Jul 6, 2000·The Journal of Clinical Investigation·L BhagatA K Saluja
May 5, 2001·The American Journal of Pathology·I A SammutM H Yacoub
Jul 24, 2001·European Journal of Immunology·M C KuppnerR D Issels
Sep 25, 2001·Circulation·J JayakumarM H Yacoub
Jan 5, 2002·Gut·J-L FrossardA K Saluja
Jan 18, 2002·Intensive Care Medicine·S Bruemmer-SmithS Schroeder
Feb 12, 2002·The Journal of Biological Chemistry·Alexzander AseaStuart K Calderwood
Feb 13, 2002·Circulation·Brit DybdahlAnders Sundan
May 22, 2002·Digestion·Mitsuo TashiroJohn A Williams
Jul 23, 2002·Lancet·David BrealeyMervyn Singer
Mar 19, 2003·European Journal of Clinical Investigation·B TriblC Madl
May 8, 2003·Intensive Care Medicine·Harriët A VreugdenhilCobi J Heijnen
Aug 19, 2003·Shock·Hsiang-Wen ChenRei-Cheng Yang