Increased hemagglutinin content in a reassortant 2009 pandemic H1N1 influenza virus with chimeric neuraminidase containing donor A/Puerto Rico/8/34 virus transmembrane and stalk domains

Vaccine
Xianghong JingZhiping Ye

Abstract

The glycoproteins, heamagglutinin (HA) and neuraminidase (NA) of influenza virus confer host protective immune responses during vaccination, which is the most effective approach for preventing influenza-associated morbidity and mortality. Since the functional balance between the HA and NA proteins may affect viral receptor binding and replication, a pandemic influenza A virus (H1N1 pdm09), strain A/Texas/05/2009, was optimized to elevate its HA antigen content by modifying the NA gene. In this study, we have constructed two 2:6 reassortant viruses between pdmH1N1 (A/Texas/05/2009) and A/Puerto Rico/8/34 (PR8), in which the NA gene of A/Texas/05/2009 was modified to contain part of the NA gene from PR8. One chimeric NA virus has the PR8 transmembrane (TM) region (HNtm 2:6) and the other contains both the PR8 NA TM and stem regions (HNst 2:6). Using quantitative reverse phase-HPLC (RP-HPLC) analysis, we observed that the HNst2:6 virus contains a higher HA1 content than HN2:6 wild type. In addition, this mutant virus displays a higher HA1 to nucleoprotein (NP) ratio, based on gel electrophoresis densitometry analysis. Furthermore, the neuraminidase activity of purified HNst 2:6 virus is approximately 30% lower than that of HN2:6 v...Continue Reading

References

May 3, 2002·Reviews in Medical Virology·Ralf WagnerHans-Dieter Klenk
May 10, 2006·Microbes and Infection·Masanobu OhuchiReiko Ohuchi
Dec 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Audray HarrisAlasdair C Steven
Dec 8, 2009·Emerging Infectious Diseases·Carrie ReedLyn Finelli
Feb 23, 2010·The Journal of Infectious Diseases·Gary P KobingerVeronika von Messling
Jun 12, 2010·The Journal of Biological Chemistry·Steven J Gamblin, John J Skehel
Apr 12, 2011·Influenza and Other Respiratory Viruses·Gabriele Neumann, Yoshihiro Kawaoka

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Citations

Mar 11, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Jaina M PatelPeriasamy Selvaraj
Apr 14, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·John M HickeyDavid B Volkin
May 13, 2015·Influenza and Other Respiratory Viruses·Callie RidenourLi-Mei Chen
Nov 8, 2014·Journal of Virology·Diogo V da SilvaRobert Daniels
Jun 2, 2018·Rapid Communications in Mass Spectrometry : RCM·Hanzhi WuZongwei Cai
Sep 4, 2015·Nature Communications·Jihui PingYoshihiro Kawaoka

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