Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence

Innate Immunity
João-Bruno SoaresAdelino F Leite-Moreira

Abstract

We evaluated expression of TLR2, TLR4 and proinflammatory genes [NF-κB, TNF-α, cyclooxygenase-2 (COX-2)] in liver samples of patients in different stages of liver disease. Fifteen patients with unexplained transaminases elevation (reference group), 22 with viral chronic hepatitis (hepatitis group), 14 with virus-induced severe fibrosis/cirrhosis (cirrhosis group) and 10 with hepatocarcinoma (hepatocarcinoma group) were consecutively included in the study. Quantification of TLR2, TLR4, NF-κB, TNF-α and COX-2 mRNA was done by real-time RT-PCR and TLR2 and TLR4 protein expression was evaluated by immunohistochemistry. Compared with reference, TLR2 and TLR4 mRNA was increased in hepatitis (TLR2: 2.66 ± 0.69; TLR4: 3.11 ± 0.79; P < 0.05) and cirrhosis (TLR2: 2.14 ± 0.5; TLR4: 1.74 ± 0.27; P < 0.05) and decreased in hepatocarcinoma (TLR2: 0.48 ± 0.15; TLR4: 0.54 ± 0.10; P < 0.05). This associated with increased TNF-α and COX-2 mRNA in hepatitis (TNF-α: 3.24 ± 0.79; COX-2: 2.47 ± 0.36; P < 0.05) and cirrhosis (TNF-α: 1.73 ± 0.28; COX-2: 1.8 ± 0.35, P < 0.05), whereas NF-κB mRNA was increased in hepatitis (2.42 ± 0.31; P < 0.05) and unchanged in cirrhosis (1.34 ± 0.17; P = 0.3). Hepatocarcinoma presented increased COX-2 mRNA (1.63 ± 0....Continue Reading

References

Mar 1, 2003·European Journal of Gastroenterology & Hepatology·Tobias ManigoldSiegbert Rossol
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Feb 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Reiner Wiest, Guadalupe Garcia-Tsao
May 3, 2005·Biological & Pharmaceutical Bulletin·Masuhiro Nishimura, Shinsaku Naito
Dec 13, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ya-Jen ChangChing-Chow Chen
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
Nov 1, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ying-Hsien HuangJiin-Haur Chuang
Dec 26, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kumar VisvanathanStephen Locarnini
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hongjin HuangRamsey C Cheung
Jul 31, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ahmed M Elsharkawy, Derek A Mann
Oct 24, 2007·Nature Medicine·Ekihiro SekiRobert F Schwabe
May 29, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ekihiro Seki, David A Brenner
Sep 2, 2008·Blood·Bruce A Beutler
Dec 17, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jinsheng GuoScott L Friedman
Jan 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Keigo MachidaMichael M-C Lai
Mar 3, 2009·Annals of the New York Academy of Sciences·Lydia GiannitrapaniGiuseppe Montalto
Apr 11, 2009·Gut·A MencinR F Schwabe
Jan 27, 2010·World Journal of Gastroenterology : WJG·Silvia Wilson GratzHani S El-Nezami
Feb 12, 2010·Expert Opinion on Therapeutic Targets·Pedro Pimentel-NunesAdelino F Leite-Moreira
Mar 25, 2010·World Journal of Gastroenterology : WJG·Paloma Martín-SanzLisardo Boscá
May 25, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Pedro Pimentel-NunesAdelino F Leite-Moreira
Jul 27, 2010·European Journal of Clinical Investigation·Peter StärkelYves Horsmans
Feb 3, 2011·Hepatology International·João-Bruno SoaresAdelino Leite-Moreira
Apr 2, 2011·Pathology Oncology Research : POR·Pedro Pimentel-NunesMário Dinis-Ribeiro

❮ Previous
Next ❯

Citations

Jul 12, 2014·Viral Immunology·Mohammad Zare-BidakiMohammad Kazemi Arababadi
Nov 26, 2015·The American Journal of Pathology·Jennifer H BenbowLaura W Schrum
Jan 27, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Mardi M AlgandabyAshraf B Abdel-Naim
Dec 4, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Desong KongShizhong Zheng
Dec 17, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Chun-Li XuDong-Liang Yang
Aug 21, 2013·Immunological Reviews·Christopher Lupfer, Thirumala-Devi Kanneganti
Feb 3, 2016·Hepatitis Monthly·Mahdi Dehghan-ManshadiMohsen Akhondi-Meybodi
Jun 12, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chuan-tao TuShun-cai Zhang
Feb 7, 2016·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Carla VenturiChristine Sempoux
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ninghong JiangLong Sui
Oct 18, 2019·Journal of Cellular Physiology·Jianhua ZhangYu Fu
Feb 4, 2016·World Journal of Hepatology·Jorge André Gomes LopesPedro Pimentel-Nunes
Oct 13, 2018·Molecular Diagnosis & Therapy·Nilesh PandeyNeeraj Jain
Jun 9, 2017·Revista Española De Enfermedades Digestivas : Organo Oficial De La Sociedad Española De Patología Digestiva·Marta Borges CanhaPedro Pimentel-Nunes
Dec 6, 2016·World Journal of Hepatology·Safak Kiziltas
Jan 28, 2021·Acta Oncologica·Valtteri KairaluomaOlli Helminen

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
surgical resection
PCR
transgenic

Software Mentioned

SPSS

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

HIV/AIDS-Related Malignancies

HIV/AIDS infection increases the risk of non-communicable diseases common in the aged including HIV/AIDS-related malignancies. Discover the latest research in HIV/AIDS-related malignancies.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.