Increased Ifi202b/IFI16 expression stimulates adipogenesis in mice and humans

Diabetologia
Mandy StadionAnnette Schürmann

Abstract

Obesity results from a constant and complex interplay between environmental stimuli and predisposing genes. Recently, we identified the IFN-activated gene Ifi202b as the most likely gene responsible for the obesity quantitative trait locus Nob3 (New Zealand Obese [NZO] obesity 3). The aim of this study was to evaluate the effects of Ifi202b on body weight and adipose tissue biology, and to clarify the functional role of its human orthologue IFI16. The impact of Ifi202b and its human orthologue IFI16 on adipogenesis was investigated by modulating their respective expression in murine 3T3-L1 and human Simpson-Golabi-Behmel syndrome (SGBS) pre-adipocytes. Furthermore, transgenic mice overexpressing IFI202b were generated and characterised with respect to metabolic traits. In humans, expression levels of IFI16 in adipose tissue were correlated with several variables of adipocyte function. In mice, IFI202b overexpression caused obesity (Δ body weight at the age of 30 weeks: 10.2 ± 1.9 g vs wild-type mice) marked by hypertrophic fat mass expansion, increased expression of Zfp423 (encoding the transcription factor zinc finger protein [ZFP] 423) and white-selective genes (Tcf21, Tle3), and decreased expression of thermogenic genes (e.g...Continue Reading

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Citations

Apr 11, 2018·Frontiers in Endocrinology·Stefanie FruhwürthKevin Jon Williams
Mar 18, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C F P LotfiJ L Kremer
Dec 24, 2020·Physiology International·M SethA G Chaudhuri

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Methods Mentioned

BETA
ELISA
SGBS
FACS
PCR
genotyping
transgenic

Software Mentioned

ViraQuest
JMP
GraphPad
GraphPad Prism

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