Increased IL-27/IL-27R expression in association with the immunopathology of murine ocular toxoplasmosis

Parasitology Research
Xinxin TongFangli Lu

Abstract

Interleukin 27 (IL-27) is a member of the IL-6/IL-12 family, and IL-27 receptor (IL-27R) consists of WSX-1 (the IL-27Rα subunit) and the signal-transducing subunit gp130. Human and mouse mast cells (MCs) express the IL-27R. To explore the expressions of IL-27/IL-27R subunits (WSX-1 and gp130) during acute ocular toxoplasmosis (OT), we established mouse model by intraocular injection of 500 Toxoplasma gondii RH strain tachyzoites. Histopathological changes were analyzed, MCs were counted by toluidine blue staining, and tryptase+/IL-27+ MCs were examined by immunofluorescence double-staining in the eyes and cervical lymph nodes (CLNs) of T. gondii-infected mice. The mRNA expressions of IL-27p28, WSX-1, gp130, and tachyzoite specific surface antigen 1 (SAG1) in the eyes and CLNs of T. gondii-infected mice, and the expressions of WSX-1 and gp130 in the murine mastocytoma cell line P815 infected with T. gondii tachyzoites in vitro were examined by using quantitative real-time reverse transcription-polymerase chain reaction. Our results showed that, after T. gondii infection, severe histopathological changes, increased numbers of total MCs and degranulated MCs, elevated expressions of IL-27p28, WSX-1, and gp130 were found in the eyes...Continue Reading

References

May 21, 1998·The Journal of Infectious Diseases·W R Henderson, E Y Chi
Jan 23, 1999·Infection and Immunity·M S HuL H Kasper
Apr 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·A MessinaA G Stewart
Jul 11, 2002·Parasitology Research·Cristiane D GilSonia M Oliani
Nov 15, 2003·Immunity·Alejandro VillarinoChristopher A Hunter
Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefan PflanzRobert A Kastelein
Aug 24, 2004·Infection and Immunity·Fangli LuLloyd H Kasper
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·David ArtisChristopher A Hunter
Jul 7, 2005·Nature Reviews. Immunology·Christopher A Hunter
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alejandro V VillarinoChristopher A Hunter
Jan 18, 2007·Immunology and Cell Biology·Pamela J Gaddi, George S Yap
Feb 7, 2007·Infection and Immunity·Elizabeth CharlesIra J Blader
May 12, 2009·Memórias do Instituto Oswaldo Cruz·Justus G Garweg, Ermanno Candolfi
May 25, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonathan S SilverChristopher A Hunter
Dec 23, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Emma C WalkerNatalie A Sims
Jul 25, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Seung Hoon BaekSo Youn Park
Oct 23, 2013·PloS One·Bo HuangFangli Lu
Aug 6, 2014·European Journal of Immunology·Aline CruzCatherine Ropert
Apr 12, 2015·Annual Review of Immunology·Hiroki Yoshida, Christopher A Hunter
Sep 21, 2015·Molecular and Cellular Biochemistry·Huai-Na QiuShiming Liu
Dec 31, 2015·European Journal of Microbiology & Immunology·Agata DukaczewskaOliver Liesenfeld
Apr 27, 2016·Klinische Monatsblätter für Augenheilkunde·J G Garweg
Aug 25, 2017·Parasite Immunology·M M MaiaUNKNOWN FAMERP and IAL Toxoplasma Research Group

❮ Previous
Next ❯

Citations

Sep 25, 2019·Infection and Immunity·Jeongho ParkChristopher A Hunter
Feb 23, 2020·International Journal of Molecular Sciences·Shanshan ZhaoGuihua Hou
Nov 15, 2018·International Archives of Allergy and Immunology·Motohiko SuzukiTamami Matsumoto

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.