Increased in vitro interleukin-12 production by peripheral blood in high-risk IDDM first degree relatives

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme
M SzelachowskaI Kinalska

Abstract

Cytokines secreted by antigen presenting cells, lymphocytes T and pancreatic beta cells are considered as the major mediators in the pathogenesis of IDDM. It has been suggested that cytokines released by macrophages/monocytes could have an initial role in beta-cell damage. The aim of the present study was the estimation of in vitro production of macrophage-derived cytokines: IL-1 beta, TNF-alpha, IL-12 by peripheral blood in high risk IDDM first degree relatives, since it could reflect early events leading to the development of type 1 diabetes in humans. The study was performed in 25 high risk IDDM subjects and 21 age and sex-matched healthy controls. IL-1 beta, TNF-alpha and IL-12 concentrations in supernatants of whole blood cultures with PHA (10 micrograms/ml) were quantified by ELISA. In the ICA positive relatives of IDDM subjects levels of IL-12 were significantly higher as compared with the control group, both at 48 h (p < 0.02) and at 72 h (p < 0.05) of incubation and positively correlated with TNF-alpha and IL-1 beta (R = 0.46, p < 0.02 and R = 0.32, p < 0.05). We did not observe statistical differences in in vitro production of TNF-alpha and IL-1 beta between the study groups. In conclusion we suggest that our findings...Continue Reading

Citations

Feb 14, 2002·Trends in Molecular Medicine·Luciano AdoriniLeonard C Harrison
Mar 24, 2004·Current Diabetes Reports·Lars Hornum, Helle Markholst
Dec 13, 2003·Autoimmunity·Chenthamarakshan VasuBellur S Prabhakar
Jun 28, 2002·Diabetes·Abdoreza Davoodi-SemiromiJin-Xiong She
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Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ilia J ElenkovRonald L Wilder

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