Increased in vitro phenol-soluble modulin production is associated with soft tissue infection source in clinical isolates of methicillin-susceptible Staphylococcus aureus

The Journal of Infection
Robert QiVance G Fowler

Abstract

Phenol-soluble modulins (PSM) are amphipathic proteins produced by Staphylococcus aureus that promote virulence, inflammatory response, and biofilm formation. We previously showed that MRSA isolates from soft tissue infection (SSTI) produced significantly higher levels of PSM than MRSA isolates from hospital-acquired pneumonia (HAP) or infective endocarditis (IE). In this investigation, we sought to validate this finding in methicillin-susceptible S. aureus (MSSA) isolates. MSSA isolates (n = 162) from patients with SSTI, HAP, and IE were matched 1:1:1 based on geographic origin of the infection to form 54 triplets (North America n = 27, Europe n = 25, Australia n = 2). All isolates underwent spa typing and were classified using eGenomics. In vitro PSM production was quantified by high-performance liquid chromatography/mass spectrometry. Fischer's Exact Test and the Kruskal-Wallis test were used for statistical analysis. Spa1 was more common in SSTI (14.81% SSTI, 3.70% HAP, 1.85% IE) (p < 0.03). Spa2 was more common in HAP (0% SSTI, 12.96% HAP, 3.70% IE) (p < 0.01). Levels of PSMα1-4 all differed significantly among the three clinical groups, with SSTI isolates producing the highest levels and IE producing the lowest levels of ...Continue Reading

References

Dec 1, 1989·Journal of Medical Microbiology·J Z JordensR J Williams
Nov 1, 1994·The Journal of Clinical Investigation·A L CheungA S Bayer
Apr 19, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J S LiG R Corey
Jun 24, 2005·JAMA : the Journal of the American Medical Association·Vance G FowlerUNKNOWN ICE Investigators
May 1, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Martin E StryjewskiUNKNOWN Assessment of Telavancin in Complicated Skin and Skin-Structure Infections Study
May 28, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rachel J Gordon, Franklin D Lowy
Dec 10, 2008·Cell Host & Microbe·M Michal PetersonHattie D Gresham
Feb 5, 2009·Pediatric Research·George Y Liu
Dec 15, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ethan RubinsteinUNKNOWN ATTAIN Study Group
Aug 17, 2011·The Journal of Infectious Diseases·Juhsien J C NienaberUNKNOWN International Collaboration on Endocarditis-Microbiology Investigators
Aug 19, 2011·The Journal of Infectious Diseases·Scott D KobayashiFrank R Deleo
Nov 28, 2012·Applied and Environmental Microbiology·Eleni TsompanidouAnnette Dreisbach
Jul 10, 2013·Annual Review of Microbiology·András N SpaanJos A G van Strijp
Jan 23, 2014·International Journal of Medical Microbiology : IJMM·Michael Otto

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Citations

Sep 15, 2016·Expert Review of Anti-infective Therapy·Stefano G GiulieriBenjamin P Howden
Dec 27, 2016·Frontiers in Cellular and Infection Microbiology·Elisabeth HodilleOana Dumitrescu

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