Increased in vivo immunological potency of HB-110, a novel therapeutic HBV DNA vaccine, by electroporation.

Experimental & Molecular Medicine
Chae Young KimByung Gee Kim

Abstract

Pulse-induced permeabilization of cellular membranes, generally referred to as electroporation (EP), has been used for years as a tool to increase macromolecule uptake in tissues, including nucleic acids, for gene therapeutic applications, and this technique has been shown to result in improved immunogenicity. In this study, we assessed the utility of EP as a tool to improve the efficacy of HB-110, a novel therapeutic DNA vaccine against chronic hepatitis B, now in phase 1 of clinical study in South Korea. The potency of HB-110 in mice was shown to be improved by EP. The rapid onset of antigen expression and higher magnitude of humoral and cellular responses in electric pulse-treated mice revealed that EP may enable a substantial reduction in the dosage of DNA vaccine required to elicit a response similar in magnitude to that achievable via conventional administration. This study also showed that EP-based vaccination at 4-week-intervals elicited a cellular immune response which was about two-fold higher than the response elicited by conventional vaccination at 2-week intervals. These results may provide a rationale to reduce the clinical dose and increase the interval between the doses in the multidose vaccination schedule. Ele...Continue Reading

References

Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K ArakiK Yamamura
Sep 22, 1998·Nature Biotechnology·H Aihara, J Miyazaki
Sep 23, 2000·Journal of Biotechnology·M SelbyJ Ulmer
Mar 21, 2001·Vaccine·S van Drunen Littel-van den HurkL A Babiuk
Jun 15, 2004·Vaccine·Gillis OttenJeffrey B Ulmer
Jul 6, 2004·Immunological Reviews·Derek T O'HaganJeffrey B Ulmer
Sep 1, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Maria J MolnarGeorge Karpati
Sep 16, 2005·Vaccine·Torunn Elisabeth TjelleRune Kjeken
Sep 16, 2006·Human Gene Therapy·Loree C Heller, Richard Heller

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Citations

Jun 3, 2011·Human Gene Therapy·Alice RochardPascal Bigey
Nov 19, 2015·Expert Opinion on Drug Delivery·Laure LambrichtGaëlle Vandermeulen
May 3, 2011·Current Opinion in Immunology·Niranjan Y Sardesai, David B Weiner
Dec 17, 2014·Medical Microbiology and Immunology·Marie-Louise MichelStanislas Pol
Nov 29, 2016·Expert Opinion on Biological Therapy·Lucyna Cova
Jun 28, 2019·The Journal of Gene Medicine·Fazhan WangXiangrong Song
Jan 25, 2017·Human Vaccines & Immunotherapeutics·Jianqiang LiJia Dou
Jul 21, 2017·Gene Therapy·H-X DengYu-Quan Wei
Apr 10, 2019·IET Nanobiotechnology·Thomas F Cronjé, Paul T Gaynor
Aug 26, 2020·Expert Review of Clinical Pharmacology·Kimia KardaniAzam Bolhassani

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