PMID: 9525613Apr 3, 1998Paper

Increased induction of apoptosis by a Sendai virus mutant is associated with attenuation of mouse pathogenicity

Journal of Virology
M ItohM Homma

Abstract

An avirulent mutant of Sendai virus, Ohita-MVC11 (MVC11), was generated from a highly virulent field strain, Ohita-M1 (M1), through successive passages in LLC-MK2 cell cultures (M. Itoh, Y. Isegawa, H. Hotta, and M. Homma, J. Gen. Virol. 78:3207-3215, 1997). In LLC-MK2 cells, MVC11 induced a high degree of apoptotic cell death that was demonstrated by chromatin condensation of the nucleus and DNA fragmentation, and production of MVC11 declined markedly after prolonged culture. On the other hand, M1 did not induce prominent apoptosis and maintained high virus titers. In primary mouse pulmonary epithelial cell cultures, M1 replicated rather slowly to reach maximum level of virus production at 3 days postinfection, and high levels of virus production were maintained thereafter without causing apoptosis. In contrast, MVC11, which produced 20 times more progeny virus than M1 at 1 day postinfection, induced a high degree of apoptotic cell death before the virus replication cycle was completed. Accordingly, the production of progeny virus was strongly inhibited thereafter. In the lungs of mice infected with MVC11, virus antigens and signals of DNA fragmentation detected by the in situ terminal deoxynucleotidyltransferase-mediated dUTP...Continue Reading

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Citations

Jun 26, 2003·The Journal of Gene Medicine·Michael BitzerWolfgang J Neubert
Apr 28, 2007·American Journal of Respiratory and Critical Care Medicine·Lance CohenUNKNOWN NHLBI Severe Asthma Research Program (SARP)
Oct 12, 2013·The Journal of General Virology·Dana N MitzelKevin S Harrod
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Oct 3, 2002·The EMBO Journal·Frédéric IseniDaniel Kolakofsky
Jan 29, 2000·The Journal of Veterinary Medical Science·D R AgungpriyonoS Tateyama
Oct 13, 2006·The Journal of General Virology·Brian D LichtyJohn C Bell
Mar 15, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·J A N Filipe, C A Gilligan
Dec 29, 2019·Scientific Reports·Gines Ávila-PérezLuis Martínez-Sobrido
Jan 31, 2021·Molecular & Cellular Proteomics : MCP·Alice MeigniéAnastassia V Komarova
Mar 20, 2002·Virus Research·Toshihiro ItoYoshihiro Kawaoka

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis