Increased inter dimeric interaction of oxy hemoglobin is necessary for attenuation of reductive pegylation promoted dissociation of tetramer

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Tao HuSeetharama A Acharya

Abstract

The propensity of site-specific carboxymethylation and propylation of Val-1(α) of Hb to attenuate the reductive hexaPEGylation-induced dissociation of tetramers has been investigated. Only reductive propylation of Val-1(α), which increases the stability of oxy Hb, attenuates the reductive hexaPEGylation-induced dissociation. Increasing the stability of the oxy conformation of Hb by chemical or genetic approaches is a strategy to generate PEGylated Hbs with native-like tetramer stability using direct PEGylation platforms. This new approach and EAF-PEGylation are the only two alternate PEGylation strategies available to design stable second-generation vasoinactive uncrosslinked PEGylated Hbs with native-like tetramer stability.

References

Apr 1, 1996·Protein Science : a Publication of the Protein Society·L R ManningJ M Manning
Jul 1, 1997·Annals of Biomedical Engineering·M Intaglietta
Sep 8, 1998·Journal of Applied Physiology·R M WinslowK D Vandegriff
Jun 6, 2000·Advanced Drug Delivery Reviews·R M Winslow
Sep 10, 2003·Journal of Protein Chemistry·Seetharama A AcharyaJoel M Friedman
Jun 25, 2008·Bioconjugate Chemistry·Fantao MengSeetharama A Acharya
Dec 17, 2008·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·K Ananda, Seetharama A Acharya
Jan 6, 2009·Biochemistry·Tao HuSeetharama A Acharya
Jan 31, 2009·Artificial Organs·Kim D Vandegriff, Robert M Winslow

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