PMID: 7540612Jun 1, 1995Paper

Increased intracellular Ca2+ selectively suppresses IL-1-induced NO production by reducing iNOS mRNA stability

The Journal of Cell Biology
Y Geng, M Lotz

Abstract

This study addresses the role of intracellular calcium (Ca2+) in the expression of iNOS, an IL-1 inducible gene in human articular chondrocytes. The calcium ionophore A23187 and ionomycin did not induce NO release or iNOS expression but inhibited dose dependently IL-1-induced NO release with IC50 of 200 nM and 100 nM, respectively. Increased intracellular Ca2+ induced by thapsigargin or cyclopiazonic acid, inhibitors of the endoplasmic reticulum Ca2+ ATPase, had similar inhibitory effects with IC50 of 1 nM and 3 microM, respectively. LPS and TNF alpha induced NO production were also suppressed by these Ca2+ elevating drugs. Levels of IL-1-induced iNOS protein were reduced by A23187, thapsigargin, and cyclopiazonic acid. These drugs as well as Bay K 8644 and KCl inhibited IL-1-induced iNOS mRNA expression. To analyze the role of Ca2+ in the expression of other IL-1 responsive genes in chondrocytes, these Ca2+ modulating drugs were tested for effects on COXII. In contrast to the inhibitory effects on iNOS mRNA, these drugs induced COXII mRNA expression and in combination with IL-1, enhanced COXII mRNA levels. Ca2+ mediated increases in COXII mRNA expression were associated with an increase in COXII protein. The kinetics of Ca2+ e...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinS H Snyder
Jun 1, 1992·Cell Calcium·H H SchmidtF Murad
Sep 4, 1992·Cell·C J Lowenstein, S H Snyder
Nov 16, 1992·Biochemical and Biophysical Research Communications·A SevernF Y Liew
Dec 16, 1991·Biochemical and Biophysical Research Communications·B Brüne, E G Lapetina
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D CaputA Cerami
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·I G CharlesS Moncada
Sep 23, 1994·Cell·C Nathan, Q W Xie
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
May 28, 1993·Biochemical and Biophysical Research Communications·R M PalmerM T Bayliss
Aug 1, 1993·The Journal of Experimental Medicine·Y VodovotzC Nathan
Aug 13, 1993·Cell·A B Sachs
Sep 15, 1993·Biochemical and Biophysical Research Communications·V BulutF Y Liew
Sep 23, 1994·Cell·H H Schmidt, U Walter
May 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S S StojilkovicF P Luyten
Aug 1, 1993·Arthritis and Rheumatism·M Stefanovic-RacicC H Evans
Sep 1, 1994·Osteoarthritis and Cartilage·J J RediskeM Lotz

❮ Previous
Next ❯

Citations

Aug 12, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·O O AkanjiT T Chowdhury
Apr 1, 2003·Animal Health Research Reviews·Robert P Hunter
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Apr 3, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M GrandolfoP D'Andrea
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R Tonon, P D'Andrea
Jun 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jan StaelensClaude Libert
Sep 14, 2007·Scandinavian Journal of Rheumatology·K VuolteenahoE Moilanen
Apr 14, 2010·Peptides·Paolo De VitoPaolo Luly
Nov 26, 2010·Cellular Microbiology·Melissa A AlturaMargaret J McFall-Ngai
May 25, 2005·Peptides·Angelika M Vollmar
May 10, 2007·Biochimica Et Biophysica Acta·Der-Zen LiuYu-Chih Liang
Feb 15, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D ReisF Espindola
Jul 17, 2004·Shock·Brian G HarbrechtBaochun Zhang
Jan 12, 2000·Oncogene·C S WilliamsR N DuBois
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ben WielockxClaude Libert
Feb 22, 2001·American Journal of Physiology. Renal Physiology·D Pérez-SalaS Lamas
May 3, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Manuel AurelianoC André Ohlin
Apr 12, 2019·International Journal of Molecular Sciences·Kaiwen MuDavid D Kitts

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
J M HeimR Gerzer
Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
M A VerbeeckJ P Burbach
© 2021 Meta ULC. All rights reserved