PMID: 11337314May 19, 2001Paper

Increased intracellular doxorubicin by anti-FAS monoclonal antibody: a mechanism that enhances the cytotoxicity in renal cell carcinoma cells

Urology
X R WuO Ogawa

Abstract

To investigate the effect of anti-Fas monoclonal antibody (mAb) on the intracellular concentration of doxorubicin in renal cell carcinoma (RCC) cells. Little is known about the influence of anti-Fas mAb on the intracellular concentration of chemotherapeutic agents. The concentration of intracellular doxorubicin was determined by high-performance liquid chromatography. The mRNA and protein levels of multidrug resistance-associated protein gene were evaluated by reverse transcriptase-polymerase chain reaction and immunocytochemistry, respectively. An increased concentration of doxorubicin inside the cells was found: 2.4-fold in ACHN cells (a human RCC cell line) after treatment with doxorubicin combined with anti-Fas mAb compared with doxorubicin alone. Of the five cases of freshly derived RCC cells treated with doxorubicin and anti-Fas mAb, the intracellular concentration of doxorubicin was increased 2.3 and 2.7-fold in two of them, respectively. Furthermore, both the mRNA and the protein levels of the multidrug resistance-associated protein gene were downregulated after treatment of ACHN cells with anti-Fas mAb. Treatment of ACHN cells with a combination of anti-Fas mAb and doxorubicin resulted in a potentiation of the doxorubi...Continue Reading

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Citations

May 1, 2002·Current Opinion in Oncology·Paul Godley, Steven W Kim
Aug 25, 2004·The Journal of Pharmacy and Pharmacology·M SawickaW Lewandowski
Mar 11, 2004·International Journal of Urology : Official Journal of the Japanese Urological Association·Xiu-Xian WuYoshiyuki Kakehi
Feb 14, 2003·International Journal of Cancer. Journal International Du Cancer·Xiu-Xian WuOsamu Ogawa

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