Increased lengthening rate decreases expression of fibroblast growth factor 2, platelet-derived growth factor, vascular endothelial growth factor, and CD31 in a rat model of distraction osteogenesis

Journal of Pediatric Orthopedics
Jonathan R SchillerMichael G Ehrlich

Abstract

The rate of lengthening has a profound impact on bone regeneration during distraction osteogenesis. Rapid distraction can delay or completely inhibit union, whereas distracting too slowly may lead to premature consolidation. However, the mechanisms responsible for retardation of healing due to rapid distraction have not been elucidated. This study explored whether rapid distraction alters the expression of certain angiogenic growth factors, in particular, fibroblast growth factor 2 (FGF-2), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF-AA), and subsequent new vessel formation as evidenced by platelet endothelial cellular adhesion marker expression (CD31), an indicator of vascular budding. Unilateral femoral lengthenings were performed in 60 male Sprague-Dawley rats using a protocol that involved a 7-day latency period and distraction rates of either 0.5 (slow distraction) or 1.5 mm/d (fast distraction) for a total of 7.0 mm of lengthening. Animals were euthanized on postoperative days 8, 10, 12, 14, and 21 (n = 6 per time point and distraction rate). Expression of FGF-2, VEGF, PDGF-AA, and CD31 was characterized immunohistochemically. Cellular staining of FGF-2, PDGF-AA, VEGF, and CD31 was redu...Continue Reading

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Citations

Apr 16, 2013·Tissue Engineering. Part B, Reviews·Asim M Makhdom, Reggie C Hamdy
Nov 19, 2011·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Xi Ling LiuLi Wu Zheng
Sep 1, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Li Wu ZhengLim Kwong Cheung
May 12, 2009·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Li Wu ZhengLim Kwong Cheung
Feb 24, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xiaowen JiangJing Hu
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Apr 12, 2012·The Journal of Trauma and Acute Care Surgery·Xi Ling LiuLi Wu Zheng
Feb 28, 2015·The Journal of Craniofacial Surgery·Guoping WuShaolan Li

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