Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans.

Scientific Reports
Vandana SampathkumarDaniel H Fine

Abstract

Aggregatibacter actinomycetemcomitans leukotoxin (LtxA) is a major virulence factor that kills leukocytes permitting it's escape from host immune surveillance. A. actinomycetemcomitans strains can produce high or low levels of toxin. Genetic differences reside in the "so called JP2" ltxA promoter region. These hyper-leukotoxin producing strains with the 530 bp deletion have been studied in detail. However, regions contained within the 530 bp deletion that could be responsible for modulation of leukotoxin production have not been defined. Here, we report, for the first time, on regions within the 530 bp that are responsible for high-levels of ltxA expression. We constructed a deletion of 530 bps in a primate isolate of A. actinomycetemcomitans, which produced leukotoxin equivalent to the JP2 strain. We then constructed sequential deletions in regions that span the 530 bps. Results indicated that expression of the ltxA transcript was reduced by a potential transcriptional terminator in promoter region 298 to 397 with a ΔG = -7.9 kcal/mol. We also confirmed previous findings that transcriptional fusion between the orfX region and ltxC increased ltxA expression. In conclusion, we constructed a hyper-leukotoxin producing A. actinomy...Continue Reading

References

Nov 27, 1987·Biochimica Et Biophysica Acta·G MenestrinaS Bhakdi
Feb 1, 1995·Advances in Dental Research·J SpitznagelD Kolodrubetz
Aug 5, 2000·Journal of Molecular Biology·M D ErmolaevaS L Salzberg
Aug 28, 2001·Nucleic Acids Research·E A LesnikD J Ecker
Mar 24, 2004·Nature Reviews. Microbiology·Douglas F Browning, Stephen J Busby
Mar 25, 2004·BioTechniques·Jehan LeeWang-Shick Ryu
Jan 18, 2006·Microbial Pathogenesis·Roger DiazScott C Kachlany
Oct 17, 2006·Journal of Bacteriology·Nataliya V BalashovaScott C Kachlany
Jul 17, 2007·International Journal of Medical Microbiology : IJMM·Vongsavanh Phongsisay, Benjamin N Fry
Mar 5, 2010·Journal of Dental Research·S C Kachlany
Aug 18, 2010·Periodontology 2000·Brian HendersonDerren Ready
Mar 29, 2011·Journal of Molecular Biology·Jason M PetersRobert Landick
Apr 12, 2011·Nature Reviews. Microbiology·Thomas J Santangelo, Irina Artsimovitch
May 18, 2011·Microbial Pathogenesis·Leigh A FeuerbacherDavid Kolodrubetz

❮ Previous
Next ❯

Citations

Nov 9, 2019·Pathogens·Georgios N BelibasakisAnders Johansson
Oct 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Senthil Kumar VelusamyDaniel H Fine
Aug 29, 2019·Journal of Clinical Medicine·Eduardo Gómez-BañuelosFelipe Andrade
Mar 20, 2021·The Japanese Dental Science Review·Akihiro YoshidaOumkeltoum Ennibi

❮ Previous
Next ❯

Datasets Mentioned

BETA
CP003496

Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

LightCycler 480
KineFold

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.