Increased level of circulating U2 small nuclear RNA fragments indicates metastasis in melanoma patients

Clinical Chemistry and Laboratory Medicine : CCLM
Jan Dominik KuhlmannGeorg Brunner

Abstract

Background: Melanoma is the most aggressive skin cancer and, despite recent advances in therapy, about 20% of the patients die of their disease. Early relapse detection and monitoring of therapy response are crucial for efficient treatment of advanced melanoma. Thus, there is a need for blood-based biomarkers in melanoma management. Serum-derived U2 small nuclear RNA fragments (RNU2-1f) were previously shown to be blood-based biomarkers for gastrointestinal and gynecologic malignancies. Here we examined whether RNU2-1f may also serve as diagnostic biomarker in advanced melanoma. Circulating RNU2-1f levels were quantified by comparative reverse transcription PCR in a training cohort of patients with metastatic melanoma (n=33, thereof regionally metastasized to skin and lymph nodes, n=23, and distantly metastasized, n=10) vs. patients with benign naevi (n=16) vs. healthy controls (n=39). RESULTS were validated in an independent patient cohort with distant metastasis (n=16) vs. controls (n=18). Circulating RNU2-1f levels in the training cohort were significantly increased in serum of regionally and distantly metastatic patients, compared with patients with benign naevi or healthy controls (p<0.0001) and allowed accurate detection ...Continue Reading

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Citations

Dec 22, 2015·Journal of Cancer Research and Clinical Oncology·Jens KöhlerAlexander Baraniskin
Nov 27, 2019·Veterinary and Comparative Oncology·Md Mahfuzur RahmanNaoki Miura
Nov 2, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Bohuslav Melichar
Feb 20, 2017·Non-coding RNA·Xiaochen XiZhi John Lu
Mar 4, 2020·Molecular Cancer·Xinyi ZhangYongguang Tao
May 4, 2021·Frontiers in Medicine·Dekker C DeaconRobert L Judson-Torres

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