Increased levels of B1 and B2 SINE transcripts in mouse fibroblast cells due to minute virus of mice infection

Virology
Warren P WilliamsC R Astell

Abstract

Minute virus of mice (MVM), an autonomous parvovirus, has served as a model for understanding parvovirus infection including host cell response to infection. In this paper, we report the effect of MVM infection on host cell gene expression in mouse fibroblast cells (LA9 cells), analyzed by differential display. Somewhat surprisingly, our data reveal that few cellular protein-coding genes appear to be up- or downregulated and identify the murine B1 and B2 short interspersed element (SINE) transcripts as being increased upon MVM infection. Primer extension assays confirm the effect of MVM infection on SINE expression and demonstrate that both SINEs are upregulated in a roughly linear fashion throughout MVM infection. They also demonstrate that the SINE response was due to RNA polymerase III transcription and not contaminating DNA or RNA polymerase II transcription. Furthermore, expression of MVM NS1, the major nonstructural protein, by transient transfection also leads to an increase in both murine SINEs. We believe this is the first time that the B1 and B2 SINEs have been shown to be altered by viral infection and the first time parvovirus infection has been shown to increase SINE expression. The increase in SINE transcripts cau...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Aug 1, 1966·Virology·L V Crawford
Oct 1, 1998·Nucleic Acids Research·C W Schmid
Feb 24, 2000·Nucleic Acids Research·M R Paule, R J White
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·A G WinterR J White
Oct 17, 2001·Journal of Virology·A Op De BeeckP Caillet-Fauquet
Nov 13, 2001·Developmental Cell·Z ZádoriP Tijssen
Jun 18, 2002·Current Opinion in Cell Biology·Alan M Weiner
Jul 24, 2002·Nucleic Acids Research·Carol M RubinCarl W Schmid
Oct 17, 2002·Genes & Development·Laura Schramm, Nouria Hernandez
Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander

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Citations

Mar 31, 2011·Cold Spring Harbor Symposia on Quantitative Biology·L L Hall, J B Lawrence
Sep 1, 2014·Virology·Pierre O Garcin, Nelly Panté
Jul 22, 2009·IUBMB Life·Ryan D WaltersJames A Goodrich
Dec 16, 2006·Genes, Chromosomes & Cancer·Christy R Hagan, Charles M Rudin
Dec 27, 2012·Journal of Proteomics·Pierre GarcinNelly Panté
Apr 18, 2016·Gene·Karina A Tatosyan, Dmitri A Kramerov
Mar 15, 2015·Virology·Pierre O GarcinNelly Panté
Feb 9, 2010·Methods : a Companion to Methods in Enzymology·Shelly AuNelly Panté
Jun 6, 2018·Biotechnology & Genetic Engineering Reviews·Christopher IsaacAthanasios Zovoilis
Sep 22, 2017·Journal of Virology·Jessica M Tucker, Britt A Glaunsinger
Nov 5, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kimberly VanheesSahar Barjesteh van Waalwijk van Doorn-Khosrovani
Mar 24, 2017·Nucleic Acids Research·John KarijolichBritt Glaunsinger

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