Increased levels of FoxA1 transcription factor in pluripotent P19 embryonal carcinoma cells stimulate neural differentiation

Stem Cells and Development
Yongjun TanYan Chen

Abstract

Transcription factor FoxA1 plays a critical role during embryonic development and is activated during retinoic acid (RA)-induced neural differentiation of pluripotent P19 embryonal carcinoma cells at the early stage, which is marked by decreased expression of Nanog and increased expression of neural stem cell marker Nestin. To further understand how FoxA1 mediates neural differentiation, we have overexpressed FoxA1 through an adenovirus vector in P19 cells and identified that early neurogenesis-related sonic hedgehog (Shh) gene is activated directly by FoxA1. Knockdown of FoxA1 expression during P19 cell neural differentiation results in prevention of Shh and Nestin induction. FoxA1 binds to Shh promoter at -486 to -462 bp region and activates the promoter in cotransfection assays. Furthermore, overexpression of FoxA1 alone in P19 cells stimulates expression of Nestin and results in decreased protein levels of Nanog. During RA-induced P19 cell differentiation, elevated levels of FoxA1 increase the population of neurons, evidenced by stimulated expression of neuron-specific Neurofilament-1 and Tubulin betaIII. Together, our results suggest a critical involvement of FoxA1 in stimulating neural differentiation of pluripotent stem ...Continue Reading

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Citations

Jan 15, 2011·Genome Research·Aurélien A SérandourJérôme Eeckhoute
Mar 13, 2012·Biochimica Et Biophysica Acta·Avin S LalmansinghAkhilesh K Nagaich
Aug 9, 2017·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yu-Qi LiXun Gong
Nov 3, 2016·Genome Research·Marco-Antonio Mendoza-ParraHinrich Gronemeyer
Apr 15, 2017·Lipids in Health and Disease·Shuixian DuShiying Xuan

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Methods Mentioned

BETA
chemical treatment
transfection
electrophoresis
flow cytometry
immunoprecipitation
ChIP
Assay
PCR

Software Mentioned

Excel

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