Increased levels of hepatic metallothionein in rat and mouse after injection of acetaminophen

Toxicology
U Wormser, D Calp

Abstract

Induction of hepatic metallothionein (MT) by acetaminophen was characterized in the rat and mouse. Treatment of rats with the hepatotoxin resulted in increase of liver MT in a dose-dependent manner. MT concentration was elevated by 41%, 140% and 260% following acetaminophen injection at doses of 250, 500 and 1000 mg/kg, respectively. The cadmium-binding protein was identified as MT by Sephadex G-75 gel filtration (Ve/Vo = 2.1). In the mouse the hepatotoxin was more potent i.e. maximal effect (increase of 230%) was achieved at the lowest applied dose (250 mg/kg). In both species maximal induction was observed 24 h post exposure and thereafter the hepatic MT content declined, indicating a relatively short half-life of the protein. The elevation of the intracellular concentration of a sulfhydryl-rich protein such as MT may serve as self protecting mechanism of the hepatocyte against highly reactive metabolites of toxic substances.

References

Jul 3, 1978·Life Sciences·M G Cherian, R A Goyer
Jan 1, 1979·Chemico-biological Interactions·K Cain, D E Holt
Oct 1, 1979·Toxicology and Applied Pharmacology·F N Kotsonis, C D Klaassen
Sep 15, 1986·Toxicology and Applied Pharmacology·N ShiraishiK Aono
Mar 15, 1987·Toxicology and Applied Pharmacology·E A BrzeznickaC D Klaassen
Jun 30, 1984·Toxicology and Applied Pharmacology·M P WaalkesC D Klaassen
Jul 1, 1984·Toxicology and Applied Pharmacology·P L Goering, C D Klaassen
Aug 15, 1980·Biochemical Pharmacology·K T Suzuki, M Yamamura
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·U WormserA Nyska

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Citations

Feb 1, 1991·Bulletin of Environmental Contamination and Toxicology·U Wormser, S BenZakine
Jan 1, 1991·Toxicology·M Kaji, H Mikawa
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·U WormserA Nyska
Mar 1, 1993·Free Radical Biology & Medicine·M Sato, I Bremner
Feb 24, 2000·Toxicology·M J Solis-HerediaA Albores
Feb 25, 2003·Protein Expression and Purification·Laura VerganiAldo Viarengo
Feb 4, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Daher Ibrahim AiboJack R Harkema
May 20, 1999·Annual Review of Pharmacology and Toxicology·C D KlaassenS Choudhuri
Oct 3, 2006·Protein Expression and Purification·Hyun ParkHye Eun Lee
Apr 16, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alexandra N HeinlothRichard S Paules
Jan 1, 1999·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals· Aldo Viarengo Bruno Burlando Francesco Dondero Anna Marro Rita Fabbri

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