Increased levels of peptidylarginine deiminase 2 in synovial fluid from anti-CCP-positive rheumatoid arthritis patients: Association with disease activity and inflammatory markers

Rheumatology
Dres DamgaardClaus Henrik Nielsen

Abstract

To quantify peptidylarginine deiminase 2 (PAD2) in SF of RA patients and OA patients, and to determine the association between PAD2 levels, disease activity and inflammatory markers in RA. Blood and SF samples were obtained from 39 RA patients and 40 patients with OA. PAD2 content and PAD activity were measured by means of in-house assays. TNF-α, IL-1β, IL-6, IL-8, IL-10 and IL-12 were measured using flow cytometry. PAD2 levels and PAD activity were higher in SF from RA than OA patients (P < 0.0001 and P = 0.03, respectively), as were all cytokine levels (P < 0.0001-0.05). SF PAD2 levels were higher among anti-CCP-positive patients than among anti-CCP-negative patients (P = 0.005). PAD2 levels in SF from RA patients correlated with disease activity, as assessed by DAS28 (P < 0.005). Moreover, SF PAD2 levels correlated with circulating CRP and anti-CCP levels (P < 0.0006), as well as with leucocyte count, IL-6, IL-8 and IL-10 levels in SF (P < 0.0001-0.02). PAD activity in SF was higher in RA patients than in OA patients, and correlated with PAD2 concentration. Extracellular PAD2 levels in SF correlate with disease activity in RA patients. Anti-CCP-positive RA patients have higher PAD2 levels in SF than anti-CCP-negative RA pati...Continue Reading

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Citations

May 7, 2016·Arthritis Research & Therapy·Dres DamgaardClaus H Nielsen
Jul 28, 2016·Arthritis Research & Therapy·Mandar BawadekarMiriam A Shelef
Nov 14, 2018·Human Molecular Genetics·Vincent A LauferS Louis Bridges
Apr 29, 2020·Nature Reviews. Rheumatology·Ashley M CurranErika Darrah
Apr 28, 2017·Arthritis Research & Therapy·Lucie Andrés CerezoLadislav Šenolt
Aug 14, 2019·Journal of Proteomics·Mandvi SharmaPer Hägglund
Mar 23, 2021·Autoimmunity Reviews·Nora PetrovskáMária Filková
Mar 31, 2021·Expert Review of Proteomics·Anne C Bay-JensenClaus H Nielsen
Nov 6, 2021·Frontiers in Immunology·María Teresa Martín MonrealDres Damgaard
Dec 11, 2021·Chemistry : a European Journal·Jennifer GrantMilan Mrksich

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