Increased life span of human osteoarthritic chondrocytes by exogenous expression of telomerase

Arthritis and Rheumatism
Sonsoles Piera-VelazquezDavidG Stokes

Abstract

To extend the life span of human osteoarthritic (OA) articular chondrocytes by introduction of the catalytic component of human telomerase while preserving the chondrocyte-specific phenotype. Human articular chondrocytes were isolated from the femoral head and tibial plateau of patients undergoing knee joint replacement for OA. The chondrocytes were cultured as monolayers and infected with a retroviral telomerase expression construct followed by selection with G418 for 10-14 days. Telomeric-repeat amplification protocol assays and telomere terminal restriction fragment length assays were performed on pools of transduced cells in order to measure telomerase activity and telomere length. Growth kinetics and population doubling capacity were assessed by passaging the cells in monolayer culture. Redifferentiation of the monolayer chondrocyte cultures was induced by transfer to suspension culture on poly-(2-hydroxyethyl-methacrylate) (polyHEMA)-coated dishes. Induction of the chondrocyte-specific phenotype was monitored by analysis of gene expression utilizing reverse transcription-polymerase chain reaction. OA chondrocytes isolated from 3 different donors (ages 41, 69, and 75 years) were transduced with a retroviral construct expre...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R C AllsoppC B Harley
Apr 1, 1992·Current Opinion in Rheumatology·A Abyad, J T Boyer
Mar 1, 1991·Mutation Research·C B Harley
Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C W Greider
May 31, 1990·Nature·C B HarleyC W Greider
Jun 1, 1983·Mechanisms of Ageing and Development·C H Evans, H I Georgescu
Oct 1, 1983·Mechanisms of Ageing and Development·M AdolpheP Lechat
Sep 1, 1995·Science·J FengJ Yu
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Jul 1, 1993·Journal of the American Geriatrics Society·D Hamerman
Oct 1, 1996·Trends in Biochemical Sciences·C Autexier, C W Greider
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J W Shay, S Bacchetti
May 5, 1998·The Anatomical Record·C S Adams, W E Horton
Aug 15, 1998·Arthritis and Rheumatism·J A Buckwalter, H J Mankin
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·V J CristofaloJ C Beck
Mar 6, 1999·The Journal of Biological Chemistry·W FuM P Mattson
Jul 8, 1999·Current Opinion in Cell Biology·T M Bryan, T R Cech
Sep 3, 1999·The Journal of Biological Chemistry·J YangG S Herron
Jan 29, 2000·Mutation Research·C Cerni
May 10, 2000·Current Opinion in Cell Biology·K Collins
Jun 6, 2000·Nature·J Y Wang
Jun 24, 2000·Nature·J WangD H Beach
Jul 13, 2000·Biochemical and Biophysical Research Communications·S SteinertW E Wright
Jul 13, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M Meyerson
Apr 3, 2001·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·J A Martin, J A Buckwalter
Mar 1, 1965·Experimental Cell Research·L HAYFLICK

❮ Previous
Next ❯

Citations

Mar 29, 2012·Modern Rheumatology·Min Wook SoBin Yoo
May 28, 2013·In Vitro Cellular & Developmental Biology. Animal·Ai-Sze WeeTunku Kamarul
Jul 29, 2003·Aging Cell·Jo S PriceIan M Clark
Sep 4, 2009·Rejuvenation Research·Michael Fossel, Barry Flanary
Aug 23, 2002·Current Opinion in Rheumatology·Thomas AignerLinda Sandell
May 15, 2007·Spine·Sylvia A ChungAshish D Diwan
Jun 16, 2011·Croatian Medical Journal·Henning Madry, Magali Cucchiarini
Jun 26, 2014·BioMed Research International·Saliya Gurusinghe, Padraig Strappe
Nov 26, 2015·Expert Opinion on Biological Therapy·Henning Madry, Magali Cucchiarini
Nov 13, 2002·Arthritis and Rheumatism·Dominik ParschJörg Fellenberg
Mar 1, 2006·Reumatología clinica·J Monfort-Faure
Feb 11, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mohamadreza Baghaban EslaminejadHouri Mivehchi
Sep 6, 2013·The Journal of Gene Medicine·Henning Madry, Magali Cucchiarini
Sep 29, 2004·Biochemical and Biophysical Research Communications·Yubo SunHerman S Cheung
Apr 1, 2006·Advanced Drug Delivery Reviews·K Gelse, H Schneider
Sep 14, 2012·The Journal of International Medical Research·D LiW Wang
Nov 27, 2008·Clinical Orthopaedics and Related Research·Chi-Chien NiuWen-Jer Chen
Jan 16, 2004·Gene Therapy·S B TrippelA J Nixon
Jul 1, 2011·Cartilage·Henning MadryMagali Cucchiarini

❮ Previous
Next ❯

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