Increased lysine transport capacity in erythrocytes from patients with chronic renal failure

Clinical Science
F C FervenzaJ C Ellory

Abstract

1. The initial rate of L-lysine influx into erythrocytes from 13 patients with chronic renal failure has been measured using 14C-labelled lysine. Ten patients were on maintenance haemodialysis and three had never been dialysed. The results are compared with data obtained from 12 normal individuals. 2. The rate of lysine influx into washed cells from buffered saline containing 0.02-0.5 mmol of L-lysine/l has been calculated. The results can be fitted with a model in which influx has a single saturable component obeying Michaelis-Menten kinetics, and a linear non-saturable component. 3. In uraemic erythrocytes the saturable component had a mean Vmax. of 0.762 mmol h-1 litre-1 of cells (n = 13, SEM 0.072) and a mean Km of 68.2 mumol/l (SEM 5.7). These values in normal erythrocytes were 0.566 mmol h-1 litre-1 of cells (n = 12, SEM 0.033) and 70.5 mumol/l (SEM 4.1), respectively. The mean apparent diffusion constant (KD) for the linear component of influx was 0.224 h-1 (SEM 0.039) in uraemic cells and 0.178 h-1 (SEM 0.028) in normals. 4. The 35% increase in mean Vmax seen in uraemic erythrocytes was statistically significant (P = 0.02). A similar increase in Vmax. in uraemic cells compared with controls was seen in erythrocytes whic...Continue Reading

Citations

Jan 20, 1994·Biotechnology and Bioengineering·L J Langsdorf, A L Zydney
Dec 1, 1990·Bioscience Reports·A FelipeX Remesar
Sep 27, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·C E Poli de FigueiredoE Barros
Dec 15, 2005·Renal Failure·Rubens Marona de OliveiraCarlos Eduardo Poli de Figueiredo
Nov 21, 2007·Nephron. Experimental Nephrology·Mariana Alves de Sá SiqueiraTatiana M C Brunini
Jul 30, 2010·Cell Biochemistry and Biophysics·Bartira Ercília Pinheiro da CostaCarlos Eduardo Poli-de-Figueiredo
Aug 1, 1994·Comparative Biochemistry and Physiology. Comparative Physiology·G Tunnicliff

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