Increased methylation of human papillomavirus type 16 DNA is associated with the severity of cervical lesions in infected females from northeast China

Oncology Letters
Wei WangQiang Ruan

Abstract

Hypermethylation of the cytosine-phosphate-guanine (CpG) sites located at the 3'-major capsid protein L1 (3'L1) and the long control region (LCR) of the human papillomavirus (HPV) genome may be associated with the progression of cervical cancer (CC). However, the methylation status of the LCR of HPV type 16 DNA remains to be elucidated in an infected Chinese population. The aim of the present study was to investigate the association between methylation of the HPV 16 L1 gene and LCR, and the severity of cervical lesions in infected female patients. Therefore, bisulfite modification, polymerase chain reaction amplification and sequencing were used to analyze 122 HPV 16-positive clinical cervical swabs obtained from patients in northeastern China. The proportion of methylated samples at each of the 7 CpG sites within the 3'-L1/5'-LCR and 5 CpG sites within the promoter region was significantly increased in patients with CC, compared with that observed in high-grade squamous intraepithelial lesions (HSIL) and normal tissue/low-grade intraepithelial lesions (LSIL) (χ2 test, P<0.01). The mean methylation frequencies of the CpG sites 7,089 and 7,143 exhibited an area under the curve value of 0.822 [95% confidence interval (CI)=0.733-0...Continue Reading

References

Mar 24, 2000·Trends in Genetics : TIG·S B Baylin, J G Herman
Jan 10, 2002·Genes & Development·Adrian Bird
Nov 9, 2002·Bioinformatics·Long-Cheng Li, Rajvir Dahiya
May 30, 2003·Molecular and Cellular Biology·Wei-Guo ZhuGregory A Otterson
Oct 14, 2004·Journal of Virology·Karl MüngerKyungwon Huh
Feb 3, 2005·International Journal of Cancer. Journal International Du Cancer·Mangalathu S RajeevanElizabeth R Unger
Aug 16, 2005·Current Opinion in Genetics & Development·François Fuks
Sep 11, 2007·Lancet·Mark SchiffmanSholom Wacholder
Sep 27, 2007·Gynecologic Oncology·Narayan ShivapurkarSophia S Wang
Jan 4, 2008·Cancer Research·Svetlana VinokurovaMagnus von Knebel Doeberitz
Aug 9, 2008·International Journal of Cancer. Journal International Du Cancer·Mina KalantariHans-Ulrich Bernard
Nov 5, 2008·Cancer·Martin A WhitesideElizabeth R Unger
Oct 13, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Dah-Ching DingTang-Yuan Chu
Aug 1, 2012·International Journal of Cancer. Journal International Du Cancer·Lisa MirabelloAttila T Lorincz
Sep 5, 2012·Chinese Journal of Cancer·Hui-Juan Yang
Oct 5, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Megan A ClarkeRobert D Burk
Oct 25, 2012·Journal of the National Cancer Institute·Nicolas WentzensenRobert D Burk
Jul 23, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Isao MurakamiDaisuke Aoki
Nov 26, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Dean BryantNed Powell
Dec 12, 2013·Obstetrics & Gynecology Science·Sun-Suk KimKyung-Un Choi
Sep 15, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Dean BryantNed Powell
Oct 5, 2014·Journal of Clinical Pathology·Lise M A De StrooperChris J L M Meijer
Nov 12, 2014·Journal of Clinical and Diagnostic Research : JCDR·Indu VermaTejinder Kaur

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Obstetrics, Gynecology, and Reproductive Biology
Dah-Ching DingTang-Yuan Chu
International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics
Jinke LiLina Hu
Zhonghua bing li xue za zhi Chinese journal of pathology
Hai-miao XuXing-hao Ni
© 2022 Meta ULC. All rights reserved