Increased microenvironment stiffness in damaged myofibers promotes myogenic progenitor cell proliferation

Skeletal Muscle
Frédéric TrenszGuillaume Grenier

Abstract

The stiffness of the myogenic stem cell microenvironment markedly influences the ability to regenerate tissue. We studied the effect of damaged myofibers on myogenic progenitor cell (MPC) proliferation and determined whether the structural integrity of the microenvironment contributes to phenotypic changes. Individual myofibers were isolated and cultured for 6 days. During this period, the cytoskeleton of myofibers and transcription factors regulating MPC differentiation were characterized by immunostaining. Atomic Force Microscopy (AFM) was performed to measure stiffness of cultured myofibers. Healthy and damaged myofibers, and their associated MPCs, were studied in skeletal muscle from dystrophic and tenotomy mouse models. MPCs were cultured on stiffness-tunable substrates, and their phenotypes were assessed by immunostaining of myogenic transcription factors. We showed that individual myofibers tend to shrink or collapse when cultured ex vivo starting from day 1 and that this is associated with a marked increase in the number of proliferative MPCs (Pax7(+)MyoD(+)). The myofibers collapsed due to a loss of viability as shown by Evans blue dye uptake and the disorganization of their cytoskeletons. Interestingly, collapsed myof...Continue Reading

References

Nov 1, 1982·The Anatomical Record·A K GulatiA A Zalewski
Apr 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A IrintchevA Wernig
Jul 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S TajbakhshM Buckingham
Oct 23, 1997·The Journal of Cell Biology·V StraubK P Campbell
Jun 23, 1998·Cell and Tissue Research·J Fridén, R L Lieber
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Nelson Osses, Enrique Brandan
Jan 22, 2003·Molecular Cell·Begoña García-AlvarezRobert C Liddington
Sep 18, 2003·Trends in Molecular Medicine·Guido Tarone, Giuseppe Lembo
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·A MAURO
Jul 28, 2004·The Journal of Cell Biology·Peter S ZammitJonathan R Beauchamp
Mar 24, 2006·Developmental Biology·Gabi SheferZipora Yablonka-Reuveni
Jun 16, 2006·Biomechanics and Modeling in Mechanobiology·S L Crick, F C-P Yin
Aug 11, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Peter S ZammitZipora Yablonka-Reuveni
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Feb 23, 2007·Acta histochemica·K PunktI B Buchwalow
Jun 2, 2007·Cell·Shihuan KuangMichael A Rudnicki
May 20, 2008·Cellular and Molecular Life Sciences : CMLS·B SjöblomK Djinović-Carugo
Sep 23, 2008·Nature·Alessandra SaccoHelen M Blau
Oct 28, 2008·Pflügers Archiv : European journal of physiology·Charles M CuerrierMichel Grandbois
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Benjamin GeigerAlexander D Bershadsky
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Ning WangDonald E Ingber
Mar 27, 2009·American Journal of Physiology. Cell Physiology·K J M BoonenM J Post
Sep 16, 2009·Differentiation; Research in Biological Diversity·Benjamin D CosgroveHelen M Blau
May 19, 2010·Developmental Biology·Sarah CalveHans-Georg Simon
Jun 4, 2010·Current Protocols in Cell Biology·Justin R Tse, Adam J Engler
Jul 7, 2010·Journal of Tissue Engineering and Regenerative Medicine·K J M BoonenM J Post
Jul 14, 2010·Journal of Cellular Biochemistry·David A LeeDan L Bader
Sep 3, 2010·American Journal of Physiology. Cell Physiology·Frédéric TrenszGuillaume Grenier
Oct 1, 2010·Advanced Materials·Matthias P Lutolf, Helen M Blau
Nov 26, 2010·Journal of Biomechanics·Gretchen A Meyer, Richard L Lieber
Mar 12, 2011·Regenerative Medicine·Dong LiFei Wang
Jul 29, 2011·Nanotechnology·Andrew R Harris, G T Charras

❮ Previous
Next ❯

Citations

Apr 21, 2017·Journal of Biomedical Materials Research. Part a·Phammela N Abarzúa-IllanesLoreto M Valenzuela
Nov 3, 2016·Journal of Applied Physiology·Koyal Garg, Marni D Boppart
Sep 12, 2017·Journal of Tissue Engineering·Patricia A RedondoUmber Cheema
Feb 7, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mingru BaiJing Xie
May 29, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joshua P NederveenGianni Parise
Nov 11, 2019·International Journal of Molecular Sciences·Chiara SassoliElisabetta Meacci
Apr 15, 2021·Advanced Biology·Che J Connon, Ricardo M Gouveia
Aug 20, 2021·Tissue Engineering. Part a·Ivan M BasurtoSteven R Caliari
Oct 14, 2021·International Journal of Molecular Sciences·Francesco Demetrio LofaroFederica Boraldi

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic force microscopy
force measurement
AFM

Software Mentioned

GraphPad Prism
Simple PCI
Matlab
Image Pro

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.