Increased microvascular permeability caused by toxic oxygen metabolites is partly reversed by exchanging the perfusate in isolated rat lungs

Acta Anaesthesiologica Scandinavica
J KjaeveL Bjertnaes

Abstract

Toxic oxygen metabolites (TOM) released from stimulated phagocytes and lung tissue have been shown to injure the pulmonary microcirculation. In the present study we evaluated microvascular injury caused by TOM in rat lungs perfused with plasma. The injury, as indicated by an increase in vascular permeability, was assessed by determining the fluid filtration rate (FFR) after paralysing the pulmonary vascular bed with papaverine (0.1 mg/ml). TOM were generated by adding xanthine oxidase (XO) (0.05-0.125 U/ml) and hypoxanthine (HX) (1 mmol/l) to the perfusate. FFR was measured before, 30 and 60 min after addition of XO and HX. The following interventions were done: 1. the H2O2-scavenger catalase, 2. substitution of the perfusate after 30 min, 3. BW 755 C, a combined lipoxygenase and cyclooxygenase inhibitor, and 4. indomethacin, a cyclooxygenase inhibitor. Addition of XO and HX caused FFR to increase from 14 +/- 4 mg/min (mean +/- s.e. mean) at the onset to 56 +/- 7 mg/min and 86 +/- 10 mg/min after 30 and 60 min, respectively. Replacing the perfusate with fresh plasma after 30 min caused a significant reduction in FFR at 60 min, from 86 +/- 11 mg/min to 58 +/- 10 mg/min. Catalase prevented the increase in FFR. Indomethacin and BW...Continue Reading

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Citations

Jul 1, 1993·Free Radical Biology & Medicine·G ValenJ Vaage
Jul 1, 1992·Acta Anaesthesiologica Scandinavica·A JolinL J Bjertnaes
Nov 11, 2005·Critical Care : the Official Journal of the Critical Care Forum·Vladimir KuklinLars Bjertnaes
Aug 1, 1996·Scandinavian Journal of Clinical and Laboratory Investigation·T SkjelbakkenJ Vaage
Dec 22, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Su-Xian YangZhao-Hui Yang

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