Increased milk yield in transgenic mice expressing insulin-like growth factor 1

Animal Biotechnology
H Y Su, Winston T K Cheng

Abstract

Insulin-like growth factor 1 (IGF-1) mediates many of the actions of growth hormone. Overexpression of IGF-1 was reported to have endocrine and paracrine/autocrine effects on somatic growth in transgenic mice. To study the paracrine/autocrine effects of IGF-1 in mammary gland, transgenic mice were produced by pronuclear microinjection of a construct containing a bovine alpha-lactalbumin (alpha-LA) promoter linked to an ovine IGF-1 cNDA. This alpha-LA promoter has previously been shown to direct expression of a human factor VIII gene specifically to the mammary gland of transgenic mice. Three transgenic mouse lines were established as a result of microinjection of 398 embryos. Transgene expression was found in mammary gland at day 1 of lactation from these three lines. Progeny test were carried out by mating two transgenic males/one transgenic female to two nontransgenic females/one nontransgenic male. Mice from one line (line 1225) were all nonexpressors and the other (line 1372) failed to produce offspring. Milk yield was analyzed in the line 1137 that produced 10 mice, of which three were transgenic females and three nontransgenic females. All of the three transgenic females showed integration of the transgene and expressed t...Continue Reading

References

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Citations

Nov 13, 2008·Journal of Mammary Gland Biology and Neoplasia·Robert A Jones, Roger A Moorehead
Feb 9, 2007·Journal of Mammary Gland Biology and Neoplasia·Darryl HadsellDaniel Torres
Jun 23, 2009·Journal of Endocrinological Investigation·F RosiD Magistrelli
Nov 13, 2015·PloS One·Mohammad Khursheed AlamTay Hui Wen

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Methods Mentioned

BETA
transgenic
biopsy
electrophoresis
PCR
in
transgenics

Software Mentioned

SYSTAT

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